Mechanism of action of D-limonene on steatosis in primary hepatocytes based on AMPK/ACC/CPT1A signaling pathway
- VernacularTitle:基于AMPK/ACC/CPT1A信号通路探讨D-柠檬烯对原代肝细胞脂肪变性的作用机制
- Author:
Qian-jun REN
1
;
Su LI
;
Yu-qing CHEN
;
Yin-ying LIAO
;
Chun-ni LIANG
;
Rui-chao FANG
;
Xu-dong LIU
;
Xiao-fang ZHAO
Author Information
- Publication Type:Journal Article
- Keywords: limonene; mouse primary hepatocytes; AMP-activated protein kinase; acetyl-CoA carboxylase 1; carnitine palmitoyltransferase 1A
- From: Chinese Pharmacological Bulletin 2025;41(9):1665-1672
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effects of D-limonene on the steatosis of primary mouse hepatocytes and its potential mechanism of action.Methods Oleic acid-induced steatosis in primary mouse hepatocytes was used as a model to observe the effects of D-limonene on cell viability,cellular lipid content,and intracellular expression of proteins such as AMP-activated protein kinase(AMPK),acetyl-coenzyme A carboxylase 1(ACC1),and carnitine palmitoyl transferase 1A(CPT1A).Results It was found that a low dose of D-limonene could effectively enhance the viability of primary mouse hepatocytes.When oleic acid at a con-centration of 300 μmol·L-1 successfully induced steatosis in primary mouse hepatocytes,D-limonene re-duced the lipid content of the cells,and D-limonene up-regulated the cellular AMPK expression level,down-regulated the cellular ACC1 and fatty acid synthetase(FAS)expression levels,which in turn promoted the overexpression of CPT1A.Conclusions D-limonene has the effect of reducing lipid deposition in primary mouse hepatocytes,and the mechanisms may be related to the activation of AMPK,the inhibitions of ACC1 and FAS,and the up-regulation of CPT1A protein expres-sion level.
