Comparison of three methods for induction of model mice with diminished ovarian reserve
10.3969/j.issn.1671-7856.2025.05.005
- VernacularTitle:三种卵巢储备功能减退小鼠模型的效果比较
- Author:
Huadan LIN
1
;
Xu WANG
;
Chunhong CHEN
;
Yuzhuo WEI
;
Yanchun LUO
;
Fanghui PANG
;
Qiuyun QIN
;
Ziyun RUAN
Author Information
1. 广西中医药大学基础医学院,南宁 530200;广西中医肝脾藏象重点研究室,南宁 530200;广西高发传染病中西医结合转化医学重点实验室,南宁 530200
- Publication Type:Journal Article
- Keywords:
diminished ovarian reserve;
Tripterygium wilfordii polyglycosides;
cyclophosphamide;
cisplatin;
animal model
- From:
Chinese Journal of Comparative Medicine
2025;35(5):51-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of Tripterygium wilfordii polyglycosides,cyclophosphamide,and cisplatin on the establishment of a mouse model of diminished ovarian reserve(DOR).Methods Mice were randomly divided into the following treatment groups:control(Ctrl),Tripterygium wilfordii polyglycosides(TWP),cyclophosphamide(CTX),and cisplatin(DDP).Mice in the TWP group received a 50 mg/kg suspension of Tripterygium wilfordii polyglycosides by gavage for 14 days,mice in the CTX group received a 20 mg/kg cyclophosphamide suspension by intraperitoneal injection for 14 days,and mice in the DDP group received a 1.5 mg/kg cisplatin solution by intraperitoneal injection for 14 days.The body weight,uterine index,and ovarian index were recorded,the estrous cycle was monitored using the vaginal smear method,and the levels of anti-Mullerian hormone(AMH),estradiol(E2),follicle stimulating hormone(FSH),and luteinizing hormone(LH)were detected using ELISA.Hematoxylin and eosin staining was used to detect ovarian follicle development.The rates of oocyte maturation and fertility were analyzed.Results The three treatment groups of mice all showed the following:significantly decreased body weight and ovarian index(P＜0.05);apparent disorder of the estrous cycle;significantly decreased levels of AMH and E2(P＜0.05);decreased and increased rates of developing follicles and atretic follicles,respectively(P＜0.05);and significantly decreased rates of oocyte maturation,pregnancy,and live birth(P＜0.05).Conclusions DOR mouse models were successfully constructed using Tripterygium wilfordii polyglycosides,cyclophosphamide,or cisplatin,as evidenced by decreased body weight and ovarian index,disordered estrous cycle and hormones,and DOR function,resulting in reduced rates of oocyte maturation,pregnancy,and total number of live births.These DOR effects were most appropriate in the cyclophosphamide group.
