circFARSA Regulates Liver Cancer Huh-7 Cell Proliferation,Migration and Invasion by Targeting miR-4319
10.3870/j.issn.1672-0741.24.04.018
- VernacularTitle:circFARSA通过靶向抑制miR-4319的表达促进Huh-7肝癌细胞增殖、迁移及侵袭
- Author:
Jinlan HE
1
;
Jian DUAN
1
;
Zhe QING
1
Author Information
1. 昆明医科大学第一附属医院普外科,昆明 650032
- Publication Type:Journal Article
- Keywords:
circFARSA;
miR-4319;
liver cancer;
proliferation;
migration;
invasion
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(1):30-35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecular mechanism of circFARSA targeting miR-4319 to modulate the prolifera-tive,migratory and invasive abilities of Huh-7 liver cancer cells.Methods The qRT-PCR method was used to detect the expres-sion of circFARSA and miR-4319 in liver cancer tissues and adjacent tissues.Liver cancer cells Huh-7 were cultured in vitro,qRT-PCR method was used to detect the expression of circFARSA and miR-4319 in Huh-7 cells.CCK-8 assay and Transwell chamber assay were used to detect proliferation,migration,and invasion abilities,respectively.Dual-luciferase reporter assay was performed to analyze the interaction between circFARSA and miR-4319.Western blot was used to detect Ki-67,MMP-2 and MM P-9 protein levels.Results The level of circFARSA was increased,and the level of miR-4319 was decreased in liver cancer tissues(bothP＜0.05).Cell viability,migration,and invasion were significantly inhibited after circFARSA silencing or miR-4319 overexpression(all P＜0.05).It was verified by double luciferase reporter gene experiment that circFARSA could competi-tively bind to miR-4319.Co-transfection of circFARSA siRNA with miR-4319 inhibitor enhanced cell viability,migration,and invasion when compared with transfection of circFARSA siRNA alone(P＜0.05).Conclusion circFARSA knockdown sup-pressed liver cancer cell tumor property by increasing the expression level of miR-4319.
