Research on Anti-inflammatory Effect of Agrin/Rapsyn on Microglia in Rats with Spinal Cord Injury
10.3870/j.issn.1672-0741.24.08.039
- VernacularTitle:Agrin/Rapsyn对脊髓损伤大鼠小胶质细胞抑炎作用的实验研究
- Author:
Xiaolu WANG
1
;
Zongsheng YIN
Author Information
1. 安徽医科大学第一附属医院骨科,合肥 230022;合肥市第一人民医院脊柱外科,合肥 230031
- Publication Type:Journal Article
- Keywords:
spinal cord injury;
Agrin;
Rapsyn;
microglia;
anti-inflammatory effect
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(1):60-66
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effects of aggregation protein(Agrin)and scaffold protein(Rapsyn)on the inflammatory responses of microglia and macrophages in injured spinal cord tissue of SD rats through activation of α4β2 nic-otinic acetylcholine receptor(α4β2 nAChR).Methods A spinal cord injury(SCI)model was established by using 4-week-old SD rats.Microglia was cultured,follo wed by incubation with Agrin and Rapsyn.Flow cytometry was utilized to detect changes in markers such as microglia and macrophages,while Co-IP was employed to detect the interaction between the two proteins.The qRT-PCR and IHC were used to identify changes in inflammatory cell marker expression in the spinal cord tissue of SCI models after lentivirus injection.Results The number of microglia and macrophages increased significantly from Day 1 to Day 14 after SCI(all P＜0.01),with the most substantial increase observed from Day 3 to Day 7.The qRT-PCR analysis revealed that in-flammatory microglial cytokine(CD68)and soluble inflammatory cytokines(IL-1β and CCL3)were upregulated after SCI.After injection of lentivirus expressing Agrin and Rapsyn,both A and R groups exhibited a slight reduction in the upregulation of these markers during the observation period of 0-1 week,whereas the A/R group demonstrated a significant reduction in their upregulation(all P＜0.01).IHC analysis indicated that the number of labeled microglial cells in the A/R group was notably lower than that in either A group or R group(all P＜0.01).Conclusion After SCI,Agrin and Rapsyn activate α4β2 nAChR and has inhibitory effects on microglia and inflammatory macrophages,which may block or slow down the progression of spinal cord injury.
