Effects of hyperbaric oxygen on the level of interleukin-1β and interleukin-10 in the lung tissue of mice following cerebral ischemia-reperfusion
10.3760/cma.j.issn.1009-6906.2014.01.004
- VernacularTitle:高压氧对脑缺血再灌注小鼠肺组织白细胞介素-1β和白细胞介素-10水平的影响
- Author:
Zhen LEI
1
;
Xingyuan JIA
;
Guozhong WANG
Author Information
1. 首都医科大学附属北京朝阳医院医学研究中心,北京,100020
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Cerebral ischemia reperfusion;
Acute lung injury;
Inflammatory cytokine;
Mice
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2014;21(1):15-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of hyperbaric oxygen (HBO) on the level of IL-1β and IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,and also to investigate their role and significance.Methods Adult male BALB/c mice were randomly divided into the ischemia-reperfusion group (or the IR group),the HBO group and the sham group.Cerebral ischemia-reperfusion model was established by clamping bilateral carotid arteries of mice.Level of IL-1β and IL-10 in the lung tissue at h 4,24 and 48 were measured respectively after cerebral ischemia-reperfusion by ELISA.Results The levels of IL-1β in the lung tissue of mice in the hyperbaric oxygen group and the ischemia-reperfusion group at h 4 were significantly higher than that of the sham group at the same time point (P ＜0.01).No statistical significance in the IL-1β levels could be seen in the lung tissue of mice between the HBO group and the ischemia-reperfusion group.The levels of IL-1β at h 24 for the HBO group and the ischemia-reperfusion group were all significantly higher than that of the sham group at the same time point (P ＜0.05,P ＜0.01).The level of IL-1β in the lung tissue of mice for the HBO group was significantly lower than that of the IR group at the same time point (P ＜ 0.05).The level of IL-10 in the lung tissue of the HBO group was significantly higher than those of the IR group and the sham group (P ＜ 0.05).Conclusions HBO could decrease the level of IL-1β and increase the level of IL-10 in the lung tissue of mice following cerebral ischemia-reperfusion,then it could offer some protection for acute lung injury induced by cerebral ischemia-reperfusion.
