Peroxynitrite promotes apoptosis of vascular endothelial cells by inducing nitration modification of hexokinase-1
10.20039/j.cnki.1007-3949.2025.11.002
- VernacularTitle:过氧亚硝酸盐通过诱导己糖激酶1硝基化修饰促进血管内皮细胞凋亡
- Author:
Hao CHEN
1
;
Shiyao ZHU
;
Xueke HE
;
Rui CHEN
;
Sikun WANG
;
Xiaomei ZHAO
;
Miao JIANG
Author Information
1. 南华大学心血管疾病研究所动脉硬化学湖南省重点实验室湖南省动脉硬化性疾病国际科技创新合作基地
- Publication Type:Journal Article
- Keywords:
peroxynitrite;
hexokinase 1;
nitration modification;
vascular endothelial cell
- From:
Chinese Journal of Arteriosclerosis
2025;33(11):930-936
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the tyrosine nitration modification of specific proteins in vascular endothelial cells and its impact on mitochondria-mediated apoptosis.Methods Human umbilical vein endothelial cells were cultured in vitro and divided into three groups:control group(treatment with dimethyl sulfoxide),3-morphansulam(SIN-1)group,and SIN-1+Fe(Ⅲ)5,10,15,20-(tetraphenyl)porphyrin(FeTPP)group.After 24 h,the levels of hexokinase 1(HK1)nitration modification,mitochondrial membrane potential,reactive oxygen species(ROS)production,and endothelial cell proliferation and apoptosis were assessed.A human umbilical vein endothelial cell line knockout of HK1 was constructed using gene editing technology,and its proliferation and apoptosis levels were detected.Results After treatment of hu-man umbilical vein endothelial cells with peroxynitrite generator SIN-1,the level of HK1 protein nitration modification sig-nificantly increased(P＜0.01),reactive oxygen species production significantly increased,mitochondrial membrane poten-tial significantly decreased,endothelial cell proliferation ability significantly decreased,and endothelial cell apoptosis level significantly increased(all P＜0.01).Peroxynitrite decomposition catalyst FeTPP could reverse the above effect(P＜0.01).In addition,HK1 gene knockout also exhibited similar antioxidant effects,with a significant decrease in endothe-lial cell proliferation ability and a significant increase in apoptosis levels(P＜0.01).Conclusion Peroxynitrite can induce an increase in the level of nitration modification of HK1 in vascular endothelial cells,which may be achieved by pro-moting the production of mitochondrial reactive oxygen species,thereby accelerating the process of endothelial cell apoptosis.
