Effects of hyperbaric oxygen combined with tangential excision surgery on local inflammatory response in early stage of degree Ⅱ burns in rats
10.3760/cma.j.issn.1009-6906.2012.06.014
- VernacularTitle:高压氧联合磨削痂术对早期大鼠深Ⅱ度烧伤局部炎症反应的影响
- Author:
Dian-fu LIU
1
;
Yong-chun WU
1
;
Yan JIANG
1
;
Hong-chun ZHOU
1
;
Rong-xia HAN
1
;
Ling YU
1
;
Hui-feng JIANG
1
;
Ting-li WANG
1
Author Information
1. 莱阳中心医院,山东省烟台,265200
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Degree Ⅱ burns;
Tangential excision;
Tissue progressive damage;
Wound healing;
Inflammatory response
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2012;19(6):364-367
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different methods for the treatment of degree Ⅱ burns surface in early stage of local inflammation and tissue damage following burns in rats.Methods Sixty healthy Wistar rats were chosen for the development of the 30％ degree Ⅱ burns model.Surgery was performed 23 hours after injury.The animals were randomly divided into the bums group (n =20),the tangential excision group (n =20) and the tangential excision combined with hyperbaric oxygen (HBO) treatment group (or the combined therapy group,n =20).Skin samples were collected at h 12,24,48,72 and d 21 after surgery.HE staining was performed to observe early tissue inflammatory response and to detect levels of malonylaldehyde (MDA) and nitric oxide (NO) in the burn surface.Results (1) Skin surface cell dehydration and coagulation necrosis were noted in the animals of the burns group.Inflammatory response was not obvious 12 hours after injury.Twenty-four hours later,inflammatory response occurred,which became more serious and expanded further 48 hours later.(2) Twenty-four hours after injury,tissue damage in the animals of the tangential excision group reached the deep dermal layer and inflammatory cell infiltration could clearly be seen,with gradual relief48 hours later.Bums injury healed 21 days later,but airs were seen.(3) For the combined therapy group,tissue damage reached the shallow dermal layer and inflammatory cell infiltration was obviously alleviated 24 hours later.Forty-eight hours later,the response band disappeared,and 21 days later,uniform but slightly sparse hair growth could be seen.The levels of NO and MDA were all lower than those of the burns group and the tangential excision group (P ＜ 0.01 or P ＜ 0.05),and returned to pre-injury levels earlier.Conclusions Tangential excision and HBO combined with tangential excision could prevent the wound surface from further infiltrating.It was shown that HBO combined with tangential excision could alleviate local inflammatory response in degree Ⅱ burns surface more significantly,and would promote healing of the wound surface.
