Effects of hyperbaric oxygen preconditioning on matrix metallo proteinases-9 and high mobility group box 1 in rats after skin flap transplantation
10.3760/cma.j.issn.1009-6906.2012.05.013
- VernacularTitle:高压氧预处理对移植皮瓣大鼠炎性介质基质金属蛋白酶-9及高迁移率蛋白B1的影响
- Author:
Xue-hua LIU
1
;
Fang LIANG
;
Ya-juan LI
;
Bao-sen PANG
;
Huan GE
;
Lian-hua WU
;
Chun-jin GAO
Author Information
1. 首都医科大学附属北京朝阳医院高压氧科, 北京,100020
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Skin flap;
MMP-9;
HMGB1
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2012;19(5):301-303,314
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of HBO preconditioning on the inflammatory reaction induced by ischemia-reperfusion following skin flap transplantation in rats.Methods Fifty-six Sprague-Dawley rats were randomly divided into 7 different groups:the sham group,the ischemia-reperfusion(IR)day 1 group,the IR day 3 group,the IR day 5 group,the HBO+ischemia-reperfusion day 1 group,the HBO+ischemiareperfusion day 3 group and the HBO+ischemia-reperfusion day 5 group.First,the abdominal pedicle skin flap model was developed and the animals were treated with HBO preconditioning.Then,levels of MMP-9 and HMGB1 were measured with ELLSA.Results The MMP-9 levels of IR d 1 and d 3 groups,the HMGB1 levels of d 1,3,5 groups were significantly higher than those of the sham group[(13.74±2.04)、(11.05±1.35)μg/L](P＜0.01).The MMP-9 level of IR d 5 group[(16.66±2.45)μg/L]was higher than that of the sham group(P＜0.05).The MMP-9 levels of the HBO+ischemia-reperfusion d 1 and d 3 groups were lower than that of the IR group on the same day(P＜0.05).The HMGB1 level of the HBO+ischemia-reperfusion d 5 group was obviously lower than that of the IR group on the same day(P＜0.01).Conclusions Hyperbaric oxygen preconditioning might alleviate inflammatory reaction induced by ischemia-reperfusion through the access of decreasing the serum levels of MMP-9 and HMGB1 in rats following skin flap transplantation.
