Advances in Structural Characterization of Protein and Polypeptide Drugs
10.3870/j.issn.1004-0781.2025.09.014
- VernacularTitle:蛋白质多肽类药物结构表征方法的研究进展
- Author:
Zijuan MAO
1
;
Yanmei XU
;
Xiaoning QIAO
;
Yanxia GAO
Author Information
1. 河北科技大学化学与制药工程学院,石家庄 050091;河北省药品医疗器械检验研究院国家药品监督管理局仿制药质量控制与评价重点实验室,石家庄 050227
- Publication Type:Journal Article
- Keywords:
Protein and polypeptide drugs;
Structural characterization;
Quality control
- From:
Herald of Medicine
2025;44(9):1465-1471
- CountryChina
- Language:Chinese
-
Abstract:
In recent years,protein and polypeptide drugs have developed rapidly and have been widely used to treat cancer,hepatitis,and other diseases.The activity of proteins and polypeptides is closely related to their structure.Protein structures can be divided into four levels:primary,secondary,tertiary,and quaternary structures.The primary structure determines the advanced structure and biological functions of proteins.To ensure drug safety,it is necessary to carry out structural characterization and quality control.The advanced structure is the basis for proteins to express their functions and activities,and changes in spatial structure can lead to alterations in functions.This article reviews the progress of research on the primary and advanced structure characterization of proteins and polypeptides,including techniques such as reversed-phase high-performance liquid chromatography,Edman degradation,mass spectrometry,and spectroscopy.It aims to provide a reference for the structural characterization and quality control of other protein and polypeptide drug products.
