Proteomics of hippocampal tissue proteins in a mice depression model which induced by lipopolysaccharide
10.3760/cma.j.issn.1006-7884.2016.04.006
- VernacularTitle:脂多糖诱导细胞因子紊乱抑郁症模型小鼠海马蛋白质组学研究
- Author:
Jianyong TANG
1
;
Chanjuan ZHOU
;
You WU
;
Wenwen LI
;
Peng XIE
Author Information
1. 400016,重庆医科大学神经科学研究中心
- Publication Type:Journal Article
- Keywords:
Depressive disorder;
Cytokines;
Hippocampus;
Proteomics;
Energy metabolism;
adenosine 5'-monoohosohate-activated Protein kinase
- From:
Chinese Journal of Psychiatry
2016;49(4):222-227
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differential proteomics of hippocampal tissue proteins and the pathogenesis in a mice depression model which induced by lipopolysaccharide (LPS).Methods According to the baseline of sucrose consumption and weight,60 male Charles River Cancer Research CD-I(R) (ICR) mice were randomly divided into depression model group and control groups (n=30 per group).After establishment of mice depression model by intraperitoneal injection of LPS,the tissue proteins were extracted from hippocampus of both groups.Subsequently,the differential proteomics analysis wasperformed by conventional two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS),and Western blotting was used to protein verification.Results (1)The experimental results from the 24 h sucrose consumption test showed consistent decreases of liquid intake (t=-8.529,P=0.008) and sucrose preference (t=-9.463,P=0.006) in the model group,forced swimming test immobility (t=3.105,P=0.003) and tail suspension test immobility (t=3.228,P=0.002) results showed obvious depressive behavior in the model group,suggesting that the mice depression model had been successfully established.(2)By 2-DE combined with mass spectrometry analysis (2DE-MS),comparing with the control group mice,47 up-regulated and 59 down-regulated proteins in the experimental group were discovered.These proteins were mainly classified into four functional categoriesincluding glutamate/γ-aminobutyric acid (GABA) metabolism,energy metabolism,synaptic transmission,cytoskeleton formation.(3)The expression level of adenosine monophosphate activated protein kinase (AMPK) which related energy metabolism was significant lower in depression model group,it was consistent with the results of 2-DE verified by Western blotting.The average grey value (-x± s) of AMPK-α 、pAMPK-α、A MPK-γ2,AMPK-β,pAMPK-β in model group relatively were (1.05±0.22),(0.84±0.17),(0.39±0.10),(0.16± 0.05),(0.66±0.38),in control group relatively were (0.98±0.14),(1.24±0.33),(0.60±0.17),(0.24±0.11),(1.53 ±0.45).The expression of pAMPK-α(t=-2.662,P=0.024),AMPK-γ2 (t=-2.491,P=0.032),and pAMPK-3(t=-3.125,P=0.005) were significant lower in model group.Conclusion The proteomics of hippocampus could significantly change and expression level of AMPK may be significant lower in cytokines disorder of depression model mice,which indicates that the pathogenesis of cytokines disorder of depression may be associated with energy metabolism disorder.
