Effect of Zhongfengting Granule on Metabolomics of Acute Cerebral Ischemia Model Rats
- VernacularTitle:中风停颗粒对急性脑缺血模型大鼠代谢组学的影响
- Author:
Zhenyu DONG
1
;
Wenhao ZHU
;
Xinyue SUN
;
Zhenhao YING
;
Xingying PU
;
Daqian GUO
;
Weiye MEN
Author Information
- Publication Type:Journal Article
- Keywords: Zhongfengting granule; Metabolomics; Acute cerebral ischemia; MCAO rat; Amino acid metabolism
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2025;27(2):564-572
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effects of Zhongfengting granule on plasma metabolites in acute cerebral ischemia MCAO model rats by non-targeted metabolomics techniques,and to elucidate the neuroprotective mechanism of Zhongfengting granule.Methods 24 male SD rats were randomly divided into blank group,model group and Chinese medicine group,with 8 rats in each group.Model group and Chinese medicine group were treated with middle cerebral artery thrombus method to establish the MCAO rat model,and the medicine was administered on the same day after the successful modeling.The Chinese medicine group was given(22.68 g·kg-1)Zhongfengting granule solution by gavage,and the blank group and model group were given equal volume normal saline for 7 days.After administration,blood was collected intravenously.Plasma samples from blank group,model group and Chinese medicine group were analyzed by ultra-high performance liquid chromatogre-quadrupole tandem time-of-flight mass spectrometer,and differential metabolites were screened by multivariate statistical analysis.The metabolic pathways of the differentiated metabolites were analyzed based on KEGG database.Results Multivariate statistical analysis showed that the content of 22 potential biomarkers in the rat model of acute cerebral ischemia had significant changes,and the above-mentioned 22 potential biomarkers were significantly reversed by Zhongfengting granule(P<0.05).The enrichment results of metabolic pathways showed that Zhongfengting granule mainly affected phenylalanine metabolism,tyrosine metabolism,ascorbic acid metabolism and arachidonic acid metabolism.Conclusion Metabolites and metabolic pathways in MCAO model rats with acute cerebral ischemia are changed,and the different metabolites and metabolic pathways can be reversed by Zhongfengting granule,which mainly involve phenylalanine metabolism and regulate energy metabolism.
