Immunomodulatory effect of myricetin on trinitrobenzene sulfonic acid-induced inflammatory bowel disease in rats
10.3969/j.issn.1000-484X.2025.08.022
- VernacularTitle:杨梅素对三硝基苯磺酸诱导的炎症性肠病大鼠的免疫调节作用
- Author:
Yani ZHOU
1
;
Ruomin WANG
;
Yun SONG
Author Information
1. 商洛学院健康管理学院,商洛 726000;商洛康养产业研究院,商洛 726000
- Publication Type:Journal Article
- Keywords:
Myricetin;
Inflammatory bowel disease;
Immune function
- From:
Chinese Journal of Immunology
2025;41(8):1928-1932,1939
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of myricetin(Myr)on trinitrobenzenesulfonic acid(TNBS)-in-duced inflammatory bowel disease(IBD)in rats.Methods:IBD rat models were established and separated into Control group,Model group,low,medium and high dose Myr[28 mg/(kg·d),Myr-L,56 mg/(kg·d),Myr-M,112 mg/(kg·d),Myr-H]groups.The dis-ease activity index(DAI)of rats was scored.Immune function indicators and colon length were measured;reagent kits were applied to measure the levels of IL-6,IL-17A,TNF-α and cAMP in serum.MPO optical density values in colon tissue was detected.HE staining was applied to observe pathological changes in colon tissue.Flow cytometry was applied to determine the proportion of Treg cells.Results:Compared with the control group,the colon tissue cells in the model group were disordered in arrangement,with a large num-ber of inflammatory cell infiltrations,obvious ulcer phenomena,a large number of cell necrosis,mucosal edema and thickening of the colon wall,DAI score,IL-6,IL-17A and TNF-α levels,spleen index,thymus index and MPO optical density value was increased sig-nificantly(P<0.05),colon length,cAMP level and the proportion of Treg cells were significantly decreased(P<0.05).Compared with the model group,the colonic tissue cells in the low-dose,medium-dose and high-dose Myr groups of rats were arranged more neatly,cAMP membrane edema and colonic wall thickening were alleviated,and inflammatory cell infiltration,cell necrosis and ulceration phenomena were reduced,DAI score,levels of IL-6,IL-17A and TNF-α,spleen index,thymus index,and MPO optical density val-ue were gradually decreased(P<0.05),while the colon length,cAMP level,and the proportion of Treg cells were significantly in-creased(P<0.05).Conclusion:Myr may inhibit inflammation levels,regulate immune function,and exert protective effects on IBD rats.
