Effect of Yinchensiling granules combined with rifaximin on gut microbiota and intestinal barrier function in mice with acute liver failure
10.3969/j.issn.1000-484X.2025.08.007
- VernacularTitle:茵陈四苓颗粒联合利福昔明对急性肝衰竭小鼠肠道菌群及肠屏障功能的影响
- Author:
Lin LYU
1
;
Jing WANG
;
Qiyao ZHANG
Author Information
1. 广安门医院济南医院(济南市中医医院)保健科,济南 250012
- Publication Type:Journal Article
- Keywords:
Acute liver failure;
Yinchensiling granules;
Rifaximin;
Intestinal microbiota;
Intestinal barrier function
- From:
Chinese Journal of Immunology
2025;41(8):1833-1839
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of Yinchensiling granules combined with rifaximin on the gut microbiota and intes-tinal barrier function in mice with acute liver failure(ALF).Methods:Fifty mice were randomly divided into Control group,model group(ALF group),Yinchensiling granules group(YCSLG group),Rifaximin group(RIF group)and Yinchensiling granules com-bined with Rifaximin group(YCSLG+RIF group),with ten mice in each group.ALF mice model were established by intraperitoneal injection of thioacetamide(TAA,300 mg/kg)twice.YCSLG group,RIF group and YCSLG+RIF group mice were orally administered Yinchensiling granules(1.5 g/kg)and rifaximin(15 mg/kg),while Control group and ALF group mice were orally administered an equal amount of 0.9%sodium chloride solution,gavage once in the morning and once in the evening for 3 consecutive days.48 hours after successful modeling,abdominal aortic blood was collected to detect liver function indicator[alanine aminotransferase(ALT),as-partate aminotransferase(AST),total bilirubin(TBIL)]levels and intestinal mucosal injury related protein[diamine oxidase(DAO),D-lactic acid(D-LA)]in mice serum;ELISA was used to detect inflammatory factor IL-1β,IL-6,TNF-α levels in serum;HE staining was used to observe pathological damage in liver and colon tissues.Immunohistochemical staining and Western blot were used to detect intestinal mucosal healing related protein[Claudin-4,Occludin,zonula occludens 1(ZO-1)]levels in colon tissue;16S rRNA sequencing was used to detect changes in gut microbiota structure in mouse feces.Results:Compared with Control group,serum ALT,AST,TBIL,IL-1β,IL-6,TNF-α,DAO and D-LA levels in ALF group were increased,Claudin-4,Occludin,ZO-1 pro-tein levels in colon tissue were decreased,the abundance of probiotics such as Lactobacillus,Dubosella,Prevotella,Akkermansia and Faecalibacterium were decreased,while the abundance of pathogenic bacteria such as Shigella,Zurich,Klebsiella,Romboutsia and Bacteroides were increased(P<0.05);compared with ALF group,serum ALT,AST,TBIL,IL-1β,IL-6,TNF-α,DAO and D-LA levels in YCSLG group,RIF group and YCSLG+RIF group were decreased,Claudin-4,Occludin,ZO-1 protein levels in colon tissue were increased,the abundance of probiotics such as Lactobacillus,Dubosella,Prevotella,Akkermansia and Faecalibacterium in feces were increased,while the abundance of pathogenic bacteria such as Shigella,Zurich,Klebsiella,Romboutsia and Bacteroides were de-creased(P<0.05);and the treatment effect of YCSLG+RIF group was better than that of YCSLG group and RIF group(P<0.05).Con-clusion:Yinchensiling granules combined with rifaximin can improve liver function,improve intestinal microbiota structure,and re-store intestinal barrier function in ALF mice.
