Effect and mechanism of glutamine enhanced enteral nutrition on Treg/Th17 balance in rats with severe acute pancreatitis
10.3969/j.issn.1000-484X.2025.08.026
- VernacularTitle:谷氨酰胺强化肠内营养对重症急性胰腺炎大鼠Treg/Th17平衡的影响及机制
- Author:
Xiaoli ZHU
1
;
Yu LIU
;
Yi ZHANG
;
Wen WANG
Author Information
1. 首都医科大学附属北京安贞医院南充医院,南充市中心医院肾脏内科,南充 637000
- Publication Type:Journal Article
- Keywords:
Glutamine;
Enteral nutrition;
Severe acute pancreatitis;
Treg/Th17 balance
- From:
Chinese Journal of Immunology
2025;41(8):1951-1957
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect and mechanism of glutamine(Gln)enhanced enteral nutrition on regulatory T cells/T helper cell 17(Treg/Th17)balance in rats with severe acute pancreatitis(SAP).Methods:Eighty rats were randomly divided into Sham group,SAP group,enteral nutrition group(EN)and Gln+EN group,with 20 rats in each group.SAP model were constructed by retrograde injection of 5%sodium taurocholate into the pancreatic duct.After 4 hours of modeling,nasojejunostomy catheterization was used to nutritional treatment,EN group and Gln+EN group were given enteral nutrition solution and added Gln enteral nutrition solution,respectively;sham group and SAP group were given equal volume of 0.9%NaCl solution.After 48 hours,HE staining was used to detect pathological damages in pancreatic tissue;ELISA was used to detect amylase,lipase,IL-6,TNF-α,IL-10 and IL-17 levels in serum;flow cytometry was used to detect Th17 and Treg cells proportion in spleen and pancreas;RT-qPCR was used to detect Foxp3,CTLA-4,GITR,RORγt,IL-17A mRNA levels in spleen;immunohistochemical staining,immunofluorescence staining,Western blot were used to detect MPO,CD68,IL-6,TNF-α,IL-10,IL-17 levels in pancreas.Results:Pancreatic structure in Sham group was normal,without bleeding or necrosis;pancreatic structure in SAP group was disrupted with significant bleeding,necrosis and inflammatory infiltration;pancreatic structure in EN group and Gln+EN group were only a small amount of bleeding,necrosis and inflammatory infiltration.Compared with Sham group,histological score,amylase and lipase levels in serum in SAP group were increased,and MPO+neutrophils and CD68+macrophages in pancreas were increased,IL-6,TNF-α,IL-17 levels in serum and pancreas were increased,IL-10 level was decreased,Th17 cell percentage,RORγt,IL-17A mRNA levels were increased,while Treg percentage,Treg/Th17,Foxp3,CTLA-4 and GITR mRNA levels were decreased(all P<0.05);compared with SAP group,histological score,amylase and lipase levels in serum in EN group and Gln+EN group were decreased,and MPO+neutrophils and CD68+macrophages in pancreas were decreased,IL-6,TNF-α,IL-17 levels in serum and pancreas were decreased,IL-10 level was increased,Th17 cell percentage,RORγt,IL-17A mRNA levels were decreased,while Treg cell percentage,Treg/Th17,Foxp3,CTLA-4 and GITR mRNA levels were increased(all P<0.05);and Gln+EN has a better therapeutic effect on SAP than EN(all P<0.05).Conclusion:Gln enhanced enteral nutrition can regulate Treg/Th17 balance in SAP rats and reduce systemic inflammatory response.
