Preliminary establishment of reference intervals for percentage and fluorescence intensity ratios of 12 platelet-leukocyte aggre-gates in circulation
10.13602/j.cnki.jcls.2025.02.11
- VernacularTitle:血液循环中12种血小板白细胞聚集体百分比和荧光强度比率参考区间的初步建立
- Author:
Tenglong DAI
1
;
Shuang LIANG
;
Bin LI
;
Cuiying LIANG
;
Xinyang YUE
;
Haiyue ZHANG
;
Jun WU
Author Information
1. 北京大学第四临床医学院,北京 102208
- Publication Type:Journal Article
- Keywords:
platelet-leukocyte aggregates;
reference intervals;
flow cytometry
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(2):130-135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the preliminary reference intervals for the percentage and fluorescence intensity ratio(FIR)for 12 platelet-leukocyte aggregates(PLAs)in peripheral blood circulation.Methods A total of 124 healthy individuals(61 males and 63 females)aged 18-90 years were selected from Beijing Jishuitan Hospital.Venous blood samples were collected in EDTA-K2 tubes for anticoagulation,and flow cytometry was used to measure the percentage and FIR of 12 types of PLAs.All the participants were grouped by gender for comparative analysis.Reference intervals for each parameter were calculated according to the WS/T 402-2024 standard.Results The percentages of platelet-T lymphocyte aggregates,platelet-CD4+T lymphocyte aggregates,and platelet-CD8+T lymphocyte aggregates in males were significantly lower than those in females(all P＜0.05).The FIRs of platelet-T lymphocyte aggregates,platelet CD8+T lymphocyte aggregates,platelet-B lymphocyte aggregates,and platelet-NK cell aggregates in males were significantly higher than those in females(all P＜0.05).Conclusion The preliminary reference intervals for the percentage and FIR of 12 types of PLAs in healthy adults have been established.Different gender may influence the detection results of platelet-leukocyte aggregates,especially platelet-lymphocyte aggregates,and the corresponding FIR values in healthy adults.Further large-scale studies should be necessary to confirm these findings.
