Effects of hyperbaric oxygen on endoplasmic reticulum stress apoptosis after acute spinal cord injury in rats
10.3760/cma.j.issn.1009-6906.2016.05.011
- VernacularTitle:高压氧治疗对急性脊髓损伤大鼠内质网应激凋亡的影响
- Author:
Xuehua LIU
1
;
Zhuo LI
;
Jing YANG
;
Fang LIANG
;
Yong WANG
;
Chunjin GAO
Author Information
1. 首都医科大学附属北京朝阳医院高压氧科,北京,100020
- Publication Type:Journal Article
- Keywords:
Spinal cord injury;
Hyperbaric oxygen;
Apoptosis;
Endoplasmic reticulum stress
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2016;23(5):372-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of hyperbaric oxygen (HBO) on endoplasmic reticulum stress(ERS) apoptosis after acute spinal cord injury (SCI) in rats,through detecting changes in the expressions of such related factor as ERS,and to further explore the mechanism of HBO biologically in the treatment of SCI.Methods The animals were randomly divided into 3 groups:the sham surgery group,the SCI group and the HBO group,each consisting of 24 rats.And each experimental group was further divided into 4 subgroups,in accordance with 1,3,7,and 14-day time-points after injury.the SCI animal model was established by using modified Allen's method,and HBO therapy was implemented accordingly.The motion of hinder limb was evaluated by using the Basso-Beattie-Bresnahan (BBB) score system at different time points after surgery.The injured spinal cord tissues were collected for TUNEL (TDT-mediated UTP nick-end labeling) for observation on the apoptosis of neural cells.The expressions of such ERS-related factors as glucose-regulated protein 78 (GRP78) and c-Jun NH2-terminal kinases (JNK) were detected by immunohistochemistry.The expression of caspases-3 was evaluated by enzyme-linked immunosorbent assay (ELISA).Results At day 7 and 14 after surgery,the BBB scores of the HBO group were significantly higher than those of the SCI group,and statistical significance could be noted,when comparisons were made between them(P ＜0.01).At day 3 and 7 after surgery,TUNEL positive cell rate of the HBO group was significantly higher than that of the SCI group,with statistical significance(P ＜0.05).At day 3 after surgery,the GRP78 positive cell counts of the HBO group were significantly higher than those of the SCI group,also with statistical significance(P ＜ 0.05).At day 3 and 7 after surgery,the JNK positive cell counts and the expression levels of caspase-3 of the HBO group were significantly lower than those of the SCI group,also with statistical significance(P ＜ 0.05,P ＜ 0.01).Conclusions The research results indicated that HBO therapy could elevate significantly the level of GRP78 following injury of the spinal cord,reduce the expression levels of JNK and caspase-3,alleviate ERS,decrease apoptosis of neural cells and enhance the scores of hinder limbs motor function of the SCI rats,thus promoting the recovery of the neurological function of the rats.
