Evaluation of different detection methods for decline pattern of syphilis antibody in non-congenital syphilis children
10.13602/j.cnki.jcls.2025.02.02
- VernacularTitle:不同方法检测非先天性梅毒患儿体内梅毒抗体衰减规律的应用评价
- Author:
Jingxuan XU
1
;
Wenhong PENG
;
Jiali WANG
;
Yunfang QIAN
;
Xianhua ZHAO
;
Ning LENG
;
Yong YANG
;
Lei CHU
;
Erfu XIE
Author Information
1. 丹阳市第二人民医院检验科,江苏丹阳 212300
- Publication Type:Journal Article
- Keywords:
pregnancy syphilis;
toluidine red unheated serum test;
enzyme-linked immunosorbent assay;
electrochemical lumines-cence method;
western blotting
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(2):88-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application values of different detection methods in monitoring the decline pattern of syphilis-spe-cific antibody in the non-congenital syphilis children.Methods A total of 80 non-congenital syphilis children were included in the study.The serum specimens were collected after birth,and the syphilis-specific antibodies were detected using electrochemilumines-cence immunoassay(ECLIA),western blotting(WB),treponema pallidum particle agglutination assay(TPPA),enzyme-linked im-munosorbent assay(ELISA),and toluidine red unheated serum test(TRUST).Follow-up was conducted every three months until the positive results of ELISA and TRUST turned to negative.Results The results of ECLIA showed that the syphilis-specific antibody lev-els in the non-congenital syphilis children declined to 25％of the level at birth within 2 to 3 months,and the rate of decline was inde-pendent of the initial concentration.WB analysis indicated that the specific IgG bands in non-congenital syphilis children at birth were consistent with those of their mother,and the sequence of specific antibodies decline was as follows:TPN47,TPN15,TPN45,and TPN17.Due to methodological limitations,the absorbance values of ELISA showed no significant change during the first three months after birth when high concentrations of antibodies were present in the samples,but it showed high sensitivity in the detection for the samples with low-concentration of syphilis antibodies.The detection rates of ECLIA,TPPA,and WB were compared by using ELISA as the reference method.At birth,the detection rates of syphilis antibodies were 100％,100％,and 90％,respectively.In 3 months after birth,the detection rates were 100％,100％,and 75％.In 6 months after birth,,they were 100％,46％,and 15％.In 9 months after birth,they were 83％,33％,and 0％.The positive rate of TRUST was 17.5％at birth.and turned to negative in 3 month of follow-up.Conclusion Syphilis specific IgG antibodies may fully transferred to the fetus and decline in a predictable pattern after birth.The comprehensive analysis for the results of the four methods suggested that dynamic detection using ECLIA method could be used to pre-dict the risk of non-congenital syphilis or terminate the follow-up at 3 months,while the seroconversion detected by WB was earlier than that by TPPA,while ELISA required the longest follow-up period.
