Luotong-Xianrong Yin improves lung function in rats with idiopathic pulmonary fibrosis by inhibiting PERK/eIF2α-mediated endoplasmic re-ticulum stress
10.3969/j.issn.1000-4718.2025.08.008
- VernacularTitle:络通纤溶饮通过抑制PERK/eIF2α介导的内质网应激改善特发性肺纤维化大鼠肺功能
- Author:
Hao WANG
1
;
Yanan YAN
;
Jiepeng WANG
;
Chaoyi FANG
;
Fang FANG
Author Information
1. 河北中医药大学第一附属医院,河北 石家庄 050011;河北省中西医结合肺病研究重点实验室,河北 石家庄 050091
- Publication Type:Journal Article
- Keywords:
Luotong-Xianrong Yin;
idiopathic pulmonary fibrosis;
endoplasmic reticulum stress;
apoptosis;
lung function
- From:
Chinese Journal of Pathophysiology
2025;41(8):1523-1531
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the mechanism of Luotong-Xianrong Yin(LTXRY)in regulating endoplasmic reticulum stress(ERS)to improve lung function of rats with idiopathic pulmonary fibrosis(IPF).METHODS:Forty-eight SPF male SD rats were randomly divided into sham group,model group,LTXRY group and nintedanib group,with 12 rats in each group.The IPF rat model was prepared by intratracheal infusion of bleomycin.The rats in LTXRY group were gavaged with aqueous solution of Chinese medicine formula granules,while the rats in nintedanib group were gavaged with aqueous solution of nintedanib.Lung function was detected by awake and unrestrained animal lung function test sys-tem.The pathomorphologic change in lung tissue was observed by HE and Masson staining.The expression of α-smooth muscle actin(α-SMA)was observed by immunohistochemical(IHC)staining,and the expression of vimentin was detect-ed by Western blot.The expressions of Bax,Bcl2,cleaved caspase-3,and PERK/eIF2α signal pathway related proteins were detected by Western blot.RESULTS:Abnormal lung function and fibrotic-like changes in lung tissue were present in rats in the IPF state.After the intervention of LTXRY,the lung function of IPF rats was significantly improved(P<0.01),the degree of lung tissue damage was significantly reduced(P<0.01),the expressions of apoptosis-related pro-teins,Bax and cleaved caspase-3,were significantly decreased(P<0.05 or P<0.01),while the expression of Bcl2 was significantly increased(P<0.05).Moreover,the ERS signal pathway,PERK/eIF2α,was significantly inhibited(P<0.05 or P<0.01).CONCLUSION:LTXRY could improve lung function in IPF rats by regulating cell apoptosis,and the mechanism may be related to inhibit endoplasmic reticulum stress.
