Expression Levels of Serum RNase E,HupB of Patients with Active Pulmonary Tuberculosis and Their Relationship with Curative Effect and Prognosis
10.3969/j.issn.1671-7414.2025.01.015
- VernacularTitle:活动性肺结核患者血清中RNase E,HupB表达水平与疗效及病情预后的关系
- Author:
Fei JIA
1
;
Chao XIN
1
;
Huan ZHOU
1
Author Information
1. 陕西省结核病防治院内七科,西安 710100
- Publication Type:Journal Article
- Keywords:
active pulmonary tuberculosis;
ribonuclease E;
HupB
- From:
Journal of Modern Laboratory Medicine
2025;40(1):78-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the expression levels of serum ribonuclease E (RNase E) and HupB of patients with active pulmonary tuberculosis (APTB) and their treatment efficacy and prognosis. Methods From March 2020 to March 2023,108 patients with APTB (APTB group) and 101 patients without APTB (control group) were selected from the Tuberculosis Prevention and Treatment Hospital of Shanxi Province. All APTB patients received anti-tuberculosis treatment,and the prognosis and recurrence of the disease were statistically analyzed. Based on the control of Mycobacterium tuberculosis after treatment,the patients were divided into a conversion group (n=73) and a non-conversion group (n=35). According to the recurrence situation,patients were divided into the recurrence group (n=22) and the non-recurrence group (n=86). Serum RNase E and HupB protein levels were detected in each group. Multivariate logistic regression analysis was used to identify the factors that affect the recurrence of APTB patients,and ROC curve analysis was used to assess the value of serum RNase E and HupB in predicting ATPB recurrence. Results The levels of serum RNase E (213.02±55.16pg/ml) and HupB protein (853.26±262.19μg/ml) in the APTB group were higher than those in the control group(102.35±24.09pg/ml,412.51±103.65μg/ml),the differences were statistically significant (t=26.368,15.779,all P<0.05). Before and after treatment,the levels of serum RNase E(247.79±20.05pg/ml,239.46±18.43pg/ml) and HupB protein(970.92±71.26μg/ml,952.34±65.08μg/ml) in the non-conversion group were higher than those in the conversion group(196.35±13.46 pg/ml,121.42±10.02 pg/ml;796.85±65.43 μg/ml,512.05±33.67 μg/ml),the differences were statistically significant (t=15.761,43.137;12.570,46.417,all P<0.05). The levels of serum RNase E(250.32±16.74pg/ml) and HupB protein(991.32±102.46μg/ml) in the recurrence group were higher than those in the non-recurrence group(203.48±21.35 pg/ml,817.94±62.35 μg/ml),the differences were statistically significant (t=9.554,10.066,all P<0.05). Multivariate Logistic regression analysis showed that no outcome of treatment[OR (95％CI):3.865(1.417～10.544)],high serum RNase E[(OR(95％CI):2.330(1.096～4.956)]and HupB protein[OR (95％CI):1.702(1.144～2.534)]levels were risk factors for APTB recurrence (all P<0.05). The AUC(95％CI) predicted by the combination of RNase E and HupB for ATPB recurrence was 0.872(0.793～0.928),higher than that predicted by RNase E[0.779(0.689～0.853)]and HupB alone[0.719(0.624～0.801)],the differences were statistically significant (Z=1.981,2.125,all P<0.05). Conclusion The serum RNase E and HupB protein levels in patients with ATPB are significantly increased and are associated with disease progression and recurrence,which can serve as prognostic markers for ATPB.
