Protective effect of mangiferin on oxidative stress injury in rat bone marrow mesenchymal stem cells
- VernacularTitle:芒果苷对大鼠骨髓间充质干细胞氧化应激损伤的保护作用
- Author:
Xiaofeng LI
1
;
Duo ZHAO
1
;
Qin OUYANG
1
;
Zixiang PANG
1
;
Yuquan LI
1
;
Qianfen CHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: bone marrow mesenchymal stem cell; mangiferin; pretreatment; oxidative stress; superoxide dismutase; catalase; malondialdehyde
- From: Chinese Journal of Tissue Engineering Research 2025;29(13):2669-2674
- CountryChina
- Language:Chinese
- Abstract: BACKGROUND:Mesenchymal stem cell transplantation has a certain effect on spinal cord injury,but there are still some problems such as low survival rate and poor efficiency of cell transplantation caused by local oxidative stress environment.OBJECTIVE:To explore the protective effect of mangiferin on oxidative stress of bone marrow mesenchymal stem cells.METHODS:Rat bone marrow mesenchymal stem cells at passage 3 were added to mangiferin and incubated for 2 hours according to the gradient of concentration (0,20,40,80,and 160 μmol/L).A serum-free medium containing 400 μmol/L H2O2 was added,and a gradient concentration of mangiferin was added again and cultured for 12 and 24 hours.Rat bone marrow mesenchymal stem cells were cultured as normal control group.Cell survival was detected by MTT assay in each group.The superoxide dismutase,malondialdehyde,and catalase in the culture medium were detected in accordance with the instruction of the kit.RESULTS AND CONCLUSION:Compared with normal control group,cell viability in the H2O2 group was significantly decreased (P<0.01),and the levels of superoxide dismutase and catalase were significantly decreased (P<0.01),while the level of malondialdehyde was significantly increased (P<0.01).Compared with H2O2 group,the survival rate of cells was significantly increased (P<0.01),and the levels of superoxide dismutase and catalase were significantly increased (P<0.01),while the level of malondialdehyde was significantly decreased (P<0.01) in the mangiferin group.Mangiferin showed concentration-dependent antioxidant stress protective activity above 20 μmol/L,and no cytotoxicity below 160 μmol/L.These findings indicate that antioxidant mangiferin can increase the antioxidant activity of bone marrow mesenchymal stem cells and provide a new preconditioning strategy for bone marrow mesenchymal stem cell transplantation.
