Acupuncture inhibites hippocampal inflammation in rats with cerebral ischemia-reperfusion injury by regulating PPARβ/δ
10.16557/j.cnki.1000-7547.2025.04.010
- VernacularTitle:针刺通过调控PPARβ/δ抑制脑缺血再灌注损伤大鼠的海马炎症反应
- Author:
Jia YUAN
1
;
Yao WANG
;
Xiaoyue LIU
;
Litong CHEN
;
Kun AI
;
Haomei TIAN
;
Canming XIE
;
Chutao CHEN
Author Information
1. 湖南中医药大学针灸推拿与康复学院,长沙 410208
- Publication Type:Journal Article
- Keywords:
acupuncture;
cerebral ischemia-reperfusion injury(CIRI);
peroxisome proliferator-activated receptor beta/delta(PPARβ/δ);
inflammation;
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(4):486-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of the expression of peroxisome proliferator-activated receptor β/δ(PPARβ/δ)in the anti-inflammatory mechanism of acupuncture(AC)against cerebral ischemia-reperfusion injury(CIRI)in rat hippocampal tissue.Methods:A total of 60 SD rats were randomly divided into Sham group,CIRI group,CIRI+AC group and CIRI+AC+PPARβ/δsiRNA group.The CIRI rat model was prepared by the method of middle cerebral artery occlusion,and acupuncture treatment was used for intervention.The neurological function of rats was assessed by the modified neurological severity score(mNSS)method after 72 hours of intervention.The brain in-farction volume ratio was observed by 2,3,5-triphenyltetrazolium chloride(TTC)staining method.The pathological morphological changes of brain tissue were observed by hematoxylin-eosin(HE)staining.The expression levels of PPARβ/δ,IL-1β,and IL-10 mRNA in the hippocampal region were detected by RT-qPCR.Results:Compared with the Sham group,the mNSS scores and the brain infarction volume ratio in all modeling groups were significantly in-creased.Compared with the CIRI group,the mNSS scores and the brain infarction volume ratio in the CIRI+AC group were significantly reduced.The expression levels of PPARβ/δ and IL-10 in the hippocampus were significantly upregu-lated,while the expression level of IL-1β was significantly downregulated(P<0.01),with scattered inflammatory cell infiltration observed.Compared with the CIRI+AC group,there was no significant difference in the mNSS scores and the brain infarction volume ratio in the CIRI+AC+PPARβ/δsiRNA group.The expression level of PPARβ/δ in the hippocampus was significantly downregulated(P<0.01),while no significant difference was found in the expression level of IL-10 and IL-1β mRNA,with a small amount of inflammatory cell infiltration observed.Conclusion:Acupunc-ture ameliorates the recovery of neurological function and the ratio of cerebral infarction volume in rats with CIRI,and its mechanism may be associated with the upregulation of PPARβ/δ expression,thereby reducing inflammatory respon-ses and exerting neuroprotective effects.
