MRS2179 improves learning and memory by inhibiting P2Y1R in rats with delayed encephalopathy after acute carbon monoxide poisoning
10.16557/j.cnki.1000-7547.2025.04.007
- VernacularTitle:MRS2179通过抑制P2Y1R改善急性一氧化碳中毒迟发性脑病大鼠的学习记忆功能
- Author:
Yunxia WANG
1
;
Wenping XIANG
;
Hui XUE
;
Miao KUAI
;
Qi YANG
;
Tianyu MENG
;
Puzhou YANG
Author Information
1. 包头医学院中心临床医学院神经内科
- Publication Type:Journal Article
- Keywords:
P2Y1 receptor(P2Y1 R);
delayed encephalopathy after acute carbon monoxide poisoning(DEACMP);
astrocytes;
neuroinflammation;
cognitive impairment;
rat
- From:
Chinese Journal of Neuroanatomy
2025;41(4):461-468
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of P2Y1Receptor(P2Y1R)-activated astrocytes in delayed encephalopathy after acute carbon monoxide poisoning(DEACMP).Methods:SD rats were randomly divided into Control,DEACMP,DEACMP+DMSO,and DEACMP+MRS2179 groups.The latter three groups were exposed to static CO inhalation to establish the DEACMP model,while the Control group inhaled air.The rats in DEACMP+MRS2179 group were injec-ted with 3 μl of P2Y1R antagonist MRS2179 with concentration of 1 mmol/L via lateral ventricle,while the DEACMP+DMSO group an equal volume of DMSO.At 14 and 21 day after DEACMP model establishment,the spatial learning and memory ability of rats were observed by Morris water maze,the injury of pyramidal cells in the CA1 area of the hippo-campus was observed by HE staining.The protein levels of glial fibrillary acidic protein(GFAP)and P2Y1receptor(P2Y1R)in the hippocampal tissues of rats were detected by immunohistochemistry and Western blot.The expressions of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the hippocampal tissues of rats were detected by ELISA.Results:In comparison with the Control group,the escape latency was found to be signifi-cantly prolonged in the DEACMP group and the DEACMP+DMSO group.Furthermore,the expression levels of GFAP,P2Y1R,IL-1β,IL-6,and TNF-α were significantly increased in the pyramidal cells of the CA1 region of the hippocam-pal tissue(P<0.05).In contrast,the escape latency was significantly reduced and the expression levels of GFAP,P2Y1R,IL-1β,IL-6,and TNF-α were significantly decreased in the DEACMP+MRS2179 group when compared with the DEACMP+DMSO group(P<0.05).Conclusion:P2Y1R activates astrocytes to release inflammatory factors lead-ing to the onset of cognitive impairment in DEACMP,and inhibition of P2Y1R reduces the expression of inflammatory factors and thus improves cognitive function in DEACMP.
