Application of single-cell RNA sequencing in spinal cord injury
10.12307/2025.044
- VernacularTitle:单细胞转录组测序技术在脊髓损伤研究中的应用
- Author:
Peigeng WANG
1
;
Dongping YE
Author Information
1. 贵州医科大学,贵州省贵阳市 550025
- Publication Type:Journal Article
- Keywords:
single-cell RNA sequencing;
spinal cord injury;
sequencing technology;
sequencing analysis;
molecular subtype;
marker gene;
cellular heterogeneity;
glial cell;
neural stem cell;
neuron
- From:
Chinese Journal of Tissue Engineering Research
2025;29(13):2822-2831
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In recent years,the study of single-cell RNA sequencing technology in spinal cord injury has provided new insights into cellular and molecular heterogeneity as well as structural changes in the central nervous system after trauma.OBJECTIVE:To review the research progress of single-cell RNA sequencing technology in spinal cord injury,comprehensively and deeply expound the application of single-cell RNA sequencing technology in spinal cord injury.METHODS:A computerized system was used to search the articles published from 2009 to 2023 in PubMed,Web of Science,CNKI,and WanFang databases with the Chinese and English search terms of "single-cell RNA sequencing,spinal cord injury,sequencing technology." Articles with poor quality,repetitive content,and non-relevance were excluded,and 57 articles were finally included for review and analysis.RESULTS AND CONCLUSION:At present,the research of single-cell RNA sequencing technology in spinal cord injury can be summarized as follows:(1) Cell subsets such as microglia,astrocytes,oligodendrocytes,macrophages,B cells,neurons,and neural stem cells were identified,and specific marker genes of these subsets were identified.(2) Microglia remain permanently active after spinal cord injury and coordinate the early stages after spinal cord injury through proliferation,immunity,and homeostatic function.Astrocytes play many important functions in spinal cord injury in an activated manner,including maintaining microenvironment balance,removing necrotic tissue,forming a protective barrier,and glial scars.Both macrophages and microglia play an important role in chronic neuroinflammation following spinal cord injury.(3) Neural stem cells and neuronal subsets can self-renew after spinal cord injury.Newly discovered neuronal subsets such as SCVsx2::Hoxa7:Zfhx3 → lumbar and SCVsx2:Hoxa10 can regenerate to natural targets and facilitate the recovery of motor function.(4) The discovery of dynamic changes in cell subsets improves our understanding of the course of spinal cord injury lesions and provides new insights into the treatment of spinal cord injury at different time points.Up to now,more basic research and sufficient clinical experiments are needed to validate the results of single-cell RNA sequencing in these studies.In the future,single-cell RNA sequencing technology is expected to open a new window for the diagnosis and treatment of spinal cord injury by interdisciplinary collaboration with bioinformatics,computer science,tissue engineering,and clinical medicine.
