Plasma-activated solutions promote tumor cells'anoikis and inhibit tumor peritoneal metastasis
- VernacularTitle:等离子体活化液促进细胞失巢凋亡抑制肿瘤腹腔播散
- Author:
Tuanhe SUN
1
;
Yuyi MA
;
Tianhao MIN
;
Kaijie REN
;
Xiaoyuan DENG
;
Xueni WANG
;
Yuanchang PENG
;
Yuanyuan LIU
;
Chengxue DANG
;
Hao ZHANG
;
Wei WANG
Author Information
- Publication Type:Journal Article
- Keywords: plasma-activated solution(PAS); anoikis; peritoneal metastasis
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2025;46(1):35-42
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the application of plasma-activated solution(PAS)in the treatment of peritoneal metastasis in mice.Methods A mice model of peritoneal tumor transplantation was established,and PAS was prepared for intervention in the mice.The growth of the peritoneally transplanted tumor was assessed using in vivo imaging technology,while the apoptosis level was evaluated through flow cytometry,immunofluorescence,and Western blotting.Results At the in vitro level,there was no significant impact on tumor cell apoptosis level under adherent conditions observed when utilizing PAS(P＞0.05).Under non-adherent condition,PAS significantly augmented tumor cell apoptosis level(P＜0.05),substantially increased the proportion of deceased cells(P＜0.05),and markedly elevated intracellular total and mitochondrial reactive oxygen species levels(P＜0.05).In vivo level,using PAS following peritoneal transplanted tumor formation exhibited no noteworthy influence on peritoneal transplanted tumor growth(P＞0.05),while immediate utilization of PAS during model conducting effectively reduced abdominal tumor spread(P＜0.05).Conclusion PAS inhibits tumor peritoneal dissemination in mice by promoting tumor cell anoikis.
