Preventive effects of chelidonine on H2O2-induced inflammatory injury of IPEC-J2 cells based on transcriptome sequencing
10.16303/j.cnki.1005-4545.2025.01.11
- VernacularTitle:基于转录组学测序研究白屈菜碱缓解H2O2诱导IPEC-J2细胞炎症损伤的作用机制
- Author:
Jiarong MO
1
;
Weifeng LU
;
Nuoyi ZHANG
;
Huiying LIN
;
Chunli ZENG
;
Fu LIN
;
Jian LI
Author Information
1. 福建农林大学动物科学学院福建省兽医中药与动物保健重点实验室,福建 福州 350002
- Publication Type:Journal Article
- Keywords:
IPEC-J2 cells;
chelidonine;
H2O2;
inflammation;
transcriptome sequencing
- From:
Chinese Journal of Veterinary Science
2025;45(1):74-83,106
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this research was to investigate the mechanism of chelidonine on H2 O2-in-duced inflammatory injury in porcine intestinal epithelial cells(IPEC-J2)through transcriptome sequencing.IPEC-J2 cells in the logarithmic growth phase were divided into the blank group(K group),H2O2 group(S group)and chelidonine group(L group),with three replicates in each group.Total RNA was isolated from each group for the purpose of constructing a sequencing li-brary.The assembled data underwent functional annotation,differential gene analysis,as well as GO and KEGG enrichment analyses.qPCR was used to confirm the expression of key differentially expressed genes(DEGs),and ELISA was utilized to assess the effect of chelidonine on the permea-bility of IPEC-J2 cells.The results indicated that the sequencing data met the necessary criteria and demonstrated a strong correlation between samples.The GO functional annotation results suggest that the intervention effects of chelidonine involve biological processes such as oxidative stress re-sponse and G2/M phase transition regulation of the mitotic cycle,and are closely associated with molecular functions,such as transmembrane transport activity.The KEGG enrichment analysis indicates that following H2 O2 treatment,DEGs in IPEC-J2 cells are predominantly enriched in the p53 signaling pathway,the coagulation cascade,the FoxO signaling pathway,and various other sig-naling pathways.Following pretreatment with chelidonine,the DEGs exhibit significant enrichment in several signaling pathways related to inflammation,including the TNF signaling pathway,syn-aptic vesicle cycle,and IL-17 signaling pathway.The results of qPCR were consistent with the se-quencing results.Chelidonine has also been found to effectively inhibit LDH release,elevate GLN content,and decrease DOA content.In conclusion,it can be seen that chelidonine can reduce cell permeability and alleviate H2 O2-induced inflammatory injury in IPEC-J2 cells by modulating in-flammation-related pathways such as the TNF signaling pathway.
