Effect and mechanism of CXCL8 on the paclitaxel sensitivity of cervical cancer cells
10.11904/j.issn.1002-3070.2025.01.003
- VernacularTitle:CXCL8对宫颈癌细胞紫杉醇敏感性的影响及机制研究
- Author:
Hua ZHANG
1
;
Ibibulla NURBIA
;
Pengfei LU
;
Chunli JIA
;
Xuanxuan PEI
;
Yongxing BAO
Author Information
1. 省部共建中亚高发病成因与防治国家重点实验室,新疆医科大学第一附属医院肿瘤二科(乌鲁木齐 830000)
- Publication Type:Journal Article
- Keywords:
Cervical cancer;
CXCL8;
Paclitaxel;
Sensitivity;
Invasion
- From:
Practical Oncology Journal
2025;39(1):13-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective The objective of this study was to explore the effect of knocking down CXCL8 on the efficacy of pacli-taxel chemotherapy in cervical squamous cell carcinoma and to investigate its potential mechanism of action.Methods The Hela cell model was used to specifically inhibit CXCL8 gene expression through lentivirus-mediated RNA interference(RNAi)technology.The optimal transfection conditions were HitransG P and a multiplicity of infection(MOI)of 100.The CCK-8 assay was used to screen the optimal intervention concentration of puromycin as 1.5μg/mL.The LV-CXCL8-RNAi(3 targets)and negative control lentivirus were transfected into cells under optimal transfection conditions and set up a blank control group.The qRT-PCR assay was used to select the sh-CXCL8-13 group lentivirus as the intervention sequence and virus for subsequent experiments.The experiments were divided into the blank control group,negative control group,sh-CXCL8 group,paclitaxel group,and sh-CXCL8+paclitaxel group.The prolifer-ative activity and invasive ability of cervical cancer cells were assessed by CCK-8 and cell invasion assays.The expression of CXCL8,Bcl2,Bax,and β-actin were detected by qRT-PCR and Western blot.Results Compared with the other four groups,the proliferative and invasive ability of Hela cells was significantly reduced in the sh-CXCL8+paclitaxel group,and the difference was statistically sig-nificant(P<0.01).The qRT-PCR results showed that the expression of CXCL8,Bcl2,PIK3CB,and Akt1 genes was significantly re-duced,and the expression of Bax gene was significantly increased in the sh-CXCL8+paclitaxel group.The difference between the groups was statistically significant(P<0.001).The results of Western blot showed that the expression of CXCL8,PIK3CB,and p-Akt1 proteins was reduced in the sh-CXCL8+paclitaxel group(P<0.05).Conclusion Knocking down CXCL8 can reduce the prolif-erative and invasive capacity of Hela cells,possibly by affecting the PI3K/Akt pathway to affect the drug sensitivity of Hela cells to paclitaxel.
