Analysis of C4BPA gene polymorphism and its correlation with milk quality in Chinese Holstein cows
10.16303/j.cnki.1005-4545.2025.01.19
- VernacularTitle:中国荷斯坦奶牛C4BPA基因遗传多态性及其与乳品质的相关性分析
- Author:
Mengyun ZHU
1
;
Ping JIANG
1
;
Xuanxu CHEN
1
;
Zhongqun TANG
1
;
Haibin YU
1
;
Yanlong ZHOU
1
;
Xianghao LIU
1
;
Zhihui ZHAO
1
;
Ziwei LIN
1
Author Information
1. 学院动物遗传与分子育种实验室,广东湛江 524088)(广东海洋大学滨海农业
- Publication Type:Journal Article
- Keywords:
haplotype combination;
C4BPA gene;
linkage analysis;
milk quality
- From:
Chinese Journal of Veterinary Science
2025;45(1):138-144
- CountryChina
- Language:Chinese
-
Abstract:
The complement component 4 binding protein alpha(C4BPA)gene is the alpha chain of complement binding protein 4.As a plasma protein involved in the complement and coagulation systems,it can influence immune responses and lipid metabolism.In order to study the polymor-phism of C4BPA gene and its correlation with milk quality traits in Chinese Holstein cows,genom-ic DNA was extracted from blood samples of 92 Chinese Holstein cows,and the target fragment of C4BPA gene was amplified by PCR,and the association analysis was performed by using direct se-quencing to obtain the SNP loci and milk quality traits.The results showed that among the four SNPs found at the third intron of the C4BPA gene,I3-11 G＞A was highly significantly correlated with milk protein and urea nitrogen(P＜0.05),I3-291 T＞G was significantly correlated with lac-tose(P＜0.05),I3-374 C＞T was highly significantly correlated with lactose and urea nitrogen(P＜0.05),and I3-375 T＞G was highly significantly correlated with lactose(P＜0.05),milk pro-tein and urea nitrogen.The chi-square test values for each point indicated that the population was in genetic equilibrium.Individuals of haplotype combination H1 H1 had the highest lactose content,and haplotype combination H1H2 can be used as the best haplotype combination in the molecular selection work of dairy cows.
