Coenzyme Q10 regulates apoptosis of TM3 cells induced by bisphenol A through au-tophagy
10.16303/j.cnki.1005-4545.2025.01.13
- VernacularTitle:辅酶Q10通过自噬调控双酚A诱导的TM3细胞凋亡
- Author:
Wenzhe YANG
1
;
Tong ZHAO
;
Feilong PAN
;
Jinhao WANG
;
Fangfang CHEN
;
Wenqi SHAO
;
Shirui WANG
;
Shuchen ZHAO
;
Kexiang LIU
;
Lijia ZHAO
Author Information
1. 东北农业大学动物医学学院,黑龙江哈尔滨 150030
- Publication Type:Journal Article
- Keywords:
bisphenol A;
coenzyme Q10;
autophagy;
apoptosis;
TM3 cells
- From:
Chinese Journal of Veterinary Science
2025;45(1):91-99
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate whether the dietary supplement coenzyme Q10(CoQ10)alleviates bisphenol A(BPA)-induced mouse Leydig cell line(TM3)damage through autophagy pathway.Cell activity was measured by CCK-8 assay when treated with different concentrations of BPA for 24 h.TM3 cells were then divided into 5 groups:CON group,BPA group,Torin2 group,CQ group and BPA+CoQ10 group,with three repeats in each group.The morphology of TM3 cells were observed under inverted light microscope.Western blot was used to determine the protein ex-pression of p62 and LC3-Ⅰ/Ⅱ.The autophagy level of TM3 cells was detected by MDC cell auto-phagy staining,the mRNA expression levels of Atg7,Beclin 1,p62 and Atg5 genes were deter-mined by RT-qPCR,and the apoptosis rate of TM3 cells was detected by flow cytometry.The results showed that compared with 0 μmol/L BPA treatment group,the viability of TM3 cells de-creased significantly after 24 h treatment with 60 μmol/L BPA(P＜0.01).Compared with CON group,the number of TM3 cells markedly reduced in the BPA-treated group,the expression of au-tophagy-related proteins(p62,LC3-Ⅱ)significantly increased(P＜0.01),comparable to the CQ group.The MDC fluorescence intensity dramatically enhanced(P＜0.01),the mRNA expression levels of autophagy-related genes(Atg7,Beclin1,p62,Atg5)significantly elevated(P＜0.01),and the apoptosis rate significantly increased(P＜0.01).Compared with BPA group,the expression levels of autophagy-related genes Atg7 and Beclin1 mRNA(P＜0.05),p 62 and Atg5 mRNA(P＜0.01)in TM3 cells treated with BPA+CoQ10 significantly decreased.Moreover,the expres-sion levels of autophagy-related protein p62(P＜0.01)and LC3-Ⅱ(P＜0.05),MDC fluorescence intensity(P＜0.05)and apoptosis rate(P＜0.01)also markedly reduced.In conclusion,CoQ10 could subsequently reduce the apoptosis of TM3 cells by improving the abnormal autophagy flux induced by BPA.
