Optimization of Processing Technology of Vinegar-Processed Artemisiae Argyi Folium by Box-Behnken Design-Response Surface Method and Its Effect on Analgesic Activity
10.14148/j.issn.1672-0482.2025.1075
- VernacularTitle:Box-Behnken设计-响应面法优化醋艾叶炮制工艺及其对镇痛活性的影响
- Author:
Yongjie SHI
1
;
Shaomeng ZHANG
;
Tangqiang SUN
;
Jieli LYU
;
Laibin ZHANG
Author Information
1. 新乡医学院药学院,河南 新乡 453003
- Publication Type:Journal Article
- Keywords:
vinegar-processed Artemisiae Argyi Folium;
processing technology;
Box-Behnken design-response surface method;
total flavonoids;
total terpenes;
6-Methoxytricin;
Santamarine;
eupatilin;
Artemisolide;
analgesic activity
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(8):1075-1086
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the optimum processing technology of vinegar-processed Artemisiae Argyi Folium(AAF),and analyze the difference of index components and analgesic activity before and after processing.METHODS The method for the determination of total flavonoids and total terpenes in AAF was established with eupatilin and Santamarine as control substances,re-spectively.The HPLC-VWD method was established for the simultaneous determination of 6-Methoxytricin,Santamarine,eupatilin and Artemisolide in AAF.Box-Behnken design-response surface method was used to optimize the processing technology of vinegar-processed AAF,with the content of total flavonoids,total terpenes and four active ingredients as the index,and the amount of rice vin-egar,moistening time,processing temperature and processing time as the factors.The quantitative analysis was combined with hierar-chical cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)to evaluate the difference of index components before and after processing.The analgesic activity before and after processing was investigated using pain mice induced by formalin.RESULTS The optimum processing parameters of vinegar-processed AAF were 16%rice vinegar dosage,moistening time 50 min,processing temperature 160℃and processing time 12 min.There were significant differences in the content of index components before and after processing.The analgesic activity of vinegar-processed AAF was signifi-cantly better than that of AAF.CONCLUSION The optimized processing technology is stable and feasible,which can provide scien-tific basis for standardizing production of vinegar-processed AAF.
