Study on the Quality Evaluation of Astragalus Membranaceus Pieces in Gansu Based on Different Extraction Methods and In-dex Component Determination
10.14148/j.issn.1672-0482.2025.1066
- VernacularTitle:基于不同提取方式及指标成分测定的甘肃黄芪饮片质量评价研究
- Author:
Ke ZHOU
1
;
Wentao CAI
;
Huimin ZHU
;
Liang NI
;
Xianlin ZHANG
;
Yi YAO
;
Shijia LIU
Author Information
1. 南京中医药大学附属医院/江苏省中医院,江苏 南京 210029;中国药科大学中药学院,江苏 南京 211198
- Publication Type:Journal Article
- Keywords:
Astragalus membranaceus;
quality evaluation;
HPLC;
Calycosin-7-O-β-D-glucopyranoside;
Astragaloside Ⅳ:standard decoction
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2025;41(8):1066-1074
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a method for determining the content of multiple components in Astragalus membranaceus,compare the content differences of Astragalus membranaceus in different regions and with different extraction methods in Gansu,and e-valuate the quality of Astragalus membranaceus from different origins of Gansu.METHODS Astragalus membranaceus samples from 40 origins in Gansu were collected on site.The content of Astragaloside Ⅳ,Calycosin-7-O-β-D-glucopyranoside,Ononin,Form-ononetin and Calycosin was determined by HPLC external standard method,and the quality differences of Astragalus membranaceus from 40 origins of Gansu were analyzed by combining bar graphs and line graphs.RESULTS Weiyuan County and Min County of Dingxi City showed high Astragaloside Ⅳ content in both extraction methods,indicating that the Astragalus membranaceus in these two regions has advantages in Astragaloside Ⅳ as an active component;for Calycosin-7-O-β-D-glucopyranoside and Ononin,although the performance of different regions in different extraction methods was different,in general,Weiyuan County and Min County of Dingxi City and Longnan City showed high content in both extraction methods.Water extraction method was more conducive to the extraction of Calycosin-7-O-β-D-glucopyranoside and Ononin,while alcohol extraction method was more conducive to the extraction of Astragalo-side Ⅳ,Calycosin and Ononin.CONCLUSION The content of chemical components of Astragalus membranaceus from different ori-gins is quite different,and the choice of extraction method has a significant effect on the content of active components,while the change trend of different compounds in the two extraction methods is basically the same.The established HPLC multi-component content de-termination method of Astragalus membranaceus is stable and reliable,which can provide a scientific basis for the quality control and comprehensive evaluation of Astragalus membranaceus medicinal materials.
