Study on the Spectral Effect Relationship of Antioxidant Activity of Mori Folium
10.3870/j.issn.1004-0781.2025.03.007
- VernacularTitle:桑叶抗氧化活性谱效关系研究
- Author:
Mengwen LIU
1
;
Qiaoling DENG
;
Jing SHEN
;
Xuanshi CHEN
;
Hui XIAO
;
Weisheng XU
Author Information
1. 新疆医科大学公共卫生学院,乌鲁木齐 830011
- Publication Type:Journal Article
- Keywords:
Mori Folium;
Fingerprints;
Spectral-effect relationship;
Antioxidant activity;
Molecular docking
- From:
Herald of Medicine
2025;44(3):387-391
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the material basis of the antioxidant activity of Mori Folium by examining the spec-trum-effect relationship.Methods High-performance liquid chromatography(HPLC)was utilized to establish the fingerprints of Mori Folium.The antioxidant activity of Mori Folium was assessed using the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging assay and other related indicators.The spectrum-effect relationship of antioxidation was analyzed using gray relational analysis,bivariate correlation analysis,and partial least squares regression analysis.Molecular docking techniques were employed to predict potential interaction targets.Results HPLC fingerprints for 13 batches of Mori Folium were established,and thirteen common peaks were marked,with similarities ranging from 0.932 to 0.998.Nine common peaks were identified by comparing them to reference substances.Differences in antioxidant activity were observed among the different batches of Mori Folium.Based on the analysis of the spectrum-effect relationship,chemical components such as chlorogenic acid,cryptochlorogenic acid,rutin,and iso-chlorogenic acid B were found to contribute significantly to the antioxidant activity.These components may exert their effects by binding to several antioxidant protein targets,such as XOD,NO-1,and PPAR-α.This implies that Mori Folium might exert its an-tioxidant action via multiple components and targets.Conclusions By integrating the fingerprint and antioxidant activity of Mori Folium,the contributions of individual components to its antioxidant activity were determined.This study provides an experi-mental basis for elucidating the substances responsible for the antioxidant activity of Mori Folium and for establishing quality con-trol methods.
