Study on the changes of intestinal flora in patients with gastric cancer and precancerous lesions and its correlation with PI3K/AKT/mTOR signal-ing pathway
10.3969/j.issn.1009-9905.2025.02.005
- VernacularTitle:胃癌及癌前病变患者肠道菌群变化及其与PI3K/AKT/mTOR信号通路相关性的研究
- Author:
Yuan LIU
1
;
Nan HOU
1
;
Jun GAO
1
Author Information
1. 南阳市第二人民医院 胃肠肿瘤科(河南 南阳 473000)
- Publication Type:Journal Article
- Keywords:
Gastric neoplasia;
Intestinal flora;
PI3K/AKT/mTOR signaling pathway
- From:
Chinese Journal of Current Advances in General Surgery
2025;28(2):119-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Exploring gut microbiota alterations in gastric cancer and precancerous lesions and their As-sociation with the PI3K/AKT/mTOR signaling pathway.Methods:According to the propensity score matching"proximity method"with a ratio of 1∶1∶1,45 patients with gastric intraepithelial neoplasia(GIN)(precancerous group),45 patients with gastric cancer(gastric cancer group),and 45 healthy individuals(health examination center)(control group)were se-lected as the research subjects.High-throughput sequencing of 16S rRNA was used to detect intestinal flora.By oper-ating the taxonomic unit(OTU),the α diversity analysis,β diversity analysis,colony structure differences,and linear dis-criminant effect analysis(LEfSe)of the three groups of intestinal flora were compared,and the PI3K/AKT/mTOR signal-ing pathway in the three groups of feces were compared.The correlation between intestinal flora and PI3K/AKT/mTOR signaling pathway was analyzed using Pearson's method.Results:A total of 572 different OTUs were obtained from fecal sample sequencing,of which 287 were shared by three groups.Principal component analysis showed that there were statistically significant differences between three groups(P＜0.05).There was no significant difference in the Chao,Shannon,Simpson,and Observed indices among the three groups(P＞0.05).The species abundance and com-position of intestinal flora in the gastric cancer group were relatively dispersed,while those in the control group were closer.The precancerous group was in the middle,and there were statistically significant differences between the three groups(P＜0.05).The LEfSe significance analysis showed that at the phylum level,the abundance of Actinobacte-ria and Firmicutes in the gastric cancer group and precancerous group was lower than that in the control group,and the gastric cancer group was lower than the precancerous group.However,the abundance of Proteobacteria,Campylobac-teria,and Bacteroidetes increased,and the gastric cancer group was higher than the precancerous group;At the genus level,the abundance of Escherichia coli-Shigella in the gastric cancer group was higher than that in the control group,while the abundance of Bacteroides was lower than that in the control group,with statistically significant differences(P＜0.05).The levels of PI3K mRNA,AKT mRNA,and mTOR mRNA in the gastric cancer group and precancerous group were higher than those in the control group,and the precancerous group was higher than the control group,with sta-tistically significant differences(P＜0.05).Pearson correlation analysis showed that Enterobacteriaceae,Escherichia coli-Shigella were positively correlated with PI3K mRNA,AKT mRNA,and mTOR mRNA,while Bacteroides was negatively correlated with them.Conclusion:The diversity,structure,and abundance of gut microbiota are associated with the occurrence and progression of precancerous lesions in gastric cancer.Enterobacteriaceae,Escherichia coli-Shigella,and PI3K/AKT/mTOR signaling pathway gene expression are all associated to some extent,which can provide a refer-ence for clinical diagnosis of precancerous lesions,understanding the development mechanism of gastric cancer,and early intervention.
