Study on anti-hepatic fibrosis effect of ferroptosis induced by ginkgoic acid in rats
10.3969/j.issn.1009-9905.2025.02.002
- VernacularTitle:银杏酸诱导铁死亡发挥抗肝纤维化效应的大鼠实验研究
- Author:
Wen-juan HAO
1
;
Sai ZHANG
1
;
Tong XIA
1
;
Kao LIU
1
Author Information
1. 山东大学齐鲁医院 器官移植科(山东 济南 250012)
- Publication Type:Journal Article
- Keywords:
Hepatic fibrosis;
Ginkgo acid;
Ferroptosis;
Rat
- From:
Chinese Journal of Current Advances in General Surgery
2025;28(2):103-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of ginkgo acid(GA)on hepatic fibrosis in rats.Methods:CCl4 and TAA were used to construct rat liver fibrosis model,and GA or combined ferroptosis inhibitor(Fer-1)was given to intervene.The liver tissue and serum of rats were obtained and histopathologically evaluated by HE,Masson and Sirius red staining,respecively.The expression of HA,LN,Ⅳ-C and PC-Ⅲ in serum were detected by Elisa and the expression of GPX4 and FTH1 were detected by Western blot.Results:HE staining showed that,com-pared with TAA and CCl4 groups,the fibrosis level of GA group was significantly decreased.The results of Masoon and Sirius red staining showed that compared with TAA and CCl4 groups,the rate of collagen fibers in liver of GA group was significantly decreased(P＜0.05).Elisa results showed that compared with TAA and CCl4 groups,the levels of HA,LN,Ⅳ-C and PC-Ⅲ in serum of rats in GA group were significantly decreased(P＜0.05).After the intervention of GA com-bined with Fer-1,Western blot results showed that compared with TAA and CCl4 groups,the expression of GPX4 and FTH1 in liver of rats in GA group were significantly increased(P＜0.05).Compared with GA group,GPX4 and FTH1 levels in Fer group were significantly decreased(P＜0.05);The histopathological results showed that compared with GA group,the rate of liver collagen fibers in Fer group was significantly increased(P＜0.05).Conclusion:GA has anti-hepatic fi-brosis effect,which is associated with inducing ferroptosis.
