Detection of G9P［4］rotavirus in the surveillance of sporadic viral diarrhea in Jiangsu province，China，in 2023
10.3760/cma.j.cn112866-20250616-00125
- VernacularTitle:江苏省2023年病毒性腹泻散发监测中发现G9P［4］型轮状病毒
- Author:
Chuchu LI
1
;
Jing AI
1
;
Yuanfang QIN
1
;
Liguo ZHU
1
;
Shenjiao WANG
1
;
Changjun BAO
1
;
Jianguang FU
1
Author Information
1. 江苏省疾病预防控制中心 卫健委肠道病原微生物重点实验室，南京 210009
- Publication Type:Journal Article
- Keywords:
Rotavirus;
Genotype;
Whole genome;
G9P［4］
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(5):611-616
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the G/P genotypes of group A rotavirus（RVA）in the 2023 sentinel surveillance in Jiangsu Province，and to conduct a molecular characterization analysis of the whole-genome sequences of four G9P［4］genotype RVA strains identified during surveillance.Methods:A total of 212 RVA-positive specimens collected from the surveillance system in 2023 were subjected to G/P genotyping using multiplex nested RT-PCR. Whole-genome sequencing was performed on six G9P［4］strains. The resulting complete genome sequences were preliminarily genotyped using BLAST，followed by comprehensive molecular characterization analyses utilizing BioEdit 7.0.5，MAFFT，MEGA 7.0，and iTOL software.Results:The overall RVA positivity rate was 6.22%. The predominant G/P combination in both outpatient and inpatient settings was G8P［8］. Among the six G9P［4］strains，four were successfully sequenced. All four exhibited the genotype constellation G9-P［4］-I2-R2-C2-M2-A2-N1-T2-E2-H2. While the NSP2 gene belonged to the N1 genotype，all other genes corresponded to the DS-1-like genogroup. Phylogenetically，the four Jiangsu G9P［4］strains clustered within Lineage V of the VP7 gene and formed a distinct minor subclade within the N1 branch of the NSP2 gene. Unique amino acid substitutions were identified at multiple VP7 neutralization antigenic epitope sites when compared to vaccine strains.Conclusions:The predominant circulating RVA strain in Jiangsu province during 2023 was G8P［8］. Concurrently，the relatively uncommon G9P［4］-N1 strain was detected. This strain exhibited significant amino acid differences at key epitopes compared to vaccine strains. Enhancing the proportion of whole-genome sequencing in RVA surveillance is warranted to obtain more detailed genetic information，thereby providing crucial data to support future vaccine development and optimization strategies.
