TLC Identification,HPLC Fingerprint and Multi-components Quantitative Analysis of Spatholobus suberectus
10.3870/j.issn.1004-0781.2025.03.021
- VernacularTitle:鸡血藤薄层色谱鉴别与高效液相色谱指纹图谱及多指标成分含量分析
- Author:
Li LI
1
;
Yi LUO
;
Huasheng SU
;
Ying MO
;
Xianjun TAN
;
Xinyi CHEN
Author Information
1. 广西壮族自治区药品检验研究院,国家药品监督管理局中药材质量监测与评价重点实验室,南宁 530021
- Publication Type:Journal Article
- Keywords:
Spatholobus suberectus;
Thin-layer chromatography;
High performance liquid chromatography fingerprints;
Chemical pattern recognition;
Multi-components;
Content determination
- From:
Herald of Medicine
2025;44(3):459-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish TLC identification method,HPLC fingerprint,chemical pattern recognition,and multi-components quantitative analysis methods of Spatholobus suberectus,and identify the quality of 16 batches of Spatholobus su-berectus from different origins.Methods The TLC method was used to identify Spatholobus suberectus qualitatively.HPLC fin-gerprint of Spatholobus suberectus was established.The quality of Spatholobus suberectus was evaluated by chemical pattern recogni-tion technologies,and the contents of Gallocatechin,protocatechuic acid,catechin,epigallocatechin and fermononetin were deter-mined by HPLC.Results TLC method of Spatholobus suberectus was established.Ten common peaks were identified in 16 bat-ches of Spatholobus suberectus,and 6 components were identified by reference substances.The similarity of fingerprint was 0.769-0.990,indicating good similarity.The samples were divided into 3 groups by cluster analysis.Results of the principal component a-nalysis showed that the top 3 samples in the list of comprehensive scores were S16、S1 1、S14.Marker compounds that cause the quality difference of Spatholobus suberectus were screened out through the orthogonal partial least squares discriminant analysis,which were fermononetin,peaks 7 and epigallocatechin.Gallocatechin,protocatechuic acid,catechin,epigallocatechin,and ferm-ononetin had a good linearity in the concentration range(r＞0.999).The content determination analysis showed that there were sig-nificant differences in the contents of the 5 index components among Spatholobus suberectus from different origins.Conclusion The established TLC and HPLC fingerprints of Spatholobus suberectus were stable and reliable,and the HPLC method for multiple active components method provides scientific support for improving the quality control method of Spatholobus suberectus.
