Effect of asiaticoside on epithelial-mesenchymal transition and radiotherapy sensitivity of esophageal cancer cells via regulation of the HIF-1α/VEGF signaling pathway
10.12007/j.issn.0258-4646.2025.02.010
- VernacularTitle:积雪草苷调节HIF-1α/VEGF信号通路对食管癌细胞上皮-间质转化和放疗敏感性的影响
- Author:
Shuangshuang LIU
1
;
Zhengshuai ZHU
1
;
Zilin YANG
1
;
Dongkui DUAN
1
;
Keyou FU
1
;
Suojiao SHEN
1
Author Information
1. 南阳市中心医院胸外科,河南南阳 473000
- Publication Type:Journal Article
- Keywords:
esophageal cancer;
asiaticoside;
hypoxia inducible factor-1α;
vascular endothelial growth factor;
epithelial-mesenchymal transition;
radiotherapy sensitivity
- From:
Journal of China Medical University
2025;54(2):150-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of asiaticoside(AS)on epithelial-mesenchymal transition(EMT)and radiotherapy sensitivity of esophageal cancer(EC)cells by its mechanism of regulating the hypoxia inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods EC9706 cells were subjected to different concentrations of AS or different doses of radiation.Methyl thiazolyl tetrazolium method was used to detect cell proliferation and calculate the half-maximal inhibitory con-centration.EC9706 cells were divided into a control group,radiology group(X-ray irradiation),AS group,combined group(AS+X-ray irradiation),and activator group(AS+X-ray irradiation+HIF-1α/VEGF pathway activator dimethyloxallyl glycine).Plate cloning experi-ments were conducted to detect sensitivity,and Transwell assays were used to detect cell migration and invasion.Flow cytometry helped detect apoptosis,and real-time quantitative polymerase chain reaction detected the expression of HIF-1α and VEGF mRNA.Western blotting method was used to detect the expression of matrix metalloproteinase-2(MMP-2),vimentin,E-cadherin,Bcl-2 associated X protein(Bax),HIF-1α,and VEGF proteins.Results With the increase of AS concentration and radiation dose,the cell viability of EC9706 cells gradually decreased;compared with the control group,the survival fraction;the numbers of cells that had migrated and invaded;the expression of HIF-1α and VEGF mRNA;and the expression of MMP-2,vimentin,HIF-1α,and VEGF in the radiology group and AS group were reduced;further,the apoptosis rate and the expression of E-cadherin and Bax were increased(P＜0.05).Compared with the radiology group and AS group,the survival fraction;the numbers of cells that had migrated and invaded;the expression of HIF-1αand VEGF mRNA;and the expressions of MMP-2,vimentin,HIF-1α,and VEGF in the combined group were reduced;the apoptosis rate and the expression of E-cadherin and Bax were increased(P＜0.05).In comparison with the combined group,the changes in the above indicators in the activator group were reversed(P＜0.05).Conclusion AS may inhibit EMT by inhibiting the HIF-1α/VEGF signaling pathway,thus enhancing the radiotherapy sensitivity of EC cells.
