Determination of three alkaloids in Xiao'er Huatan Zhike granules,including emetine,by QAMS
10.19778/j.chp.2025.04.010
- VernacularTitle:一测多评测定小儿化痰止咳颗粒中吐根碱等3种生物碱的含量
- Author:
Manyun SU
1
;
Hong LI
1
;
Jingguang LU
1
Author Information
1. 青岛市食品药品检验研究院,国家药品监督管理局海洋中药质量研究与评价重点实验室,山东青岛 266071
- Publication Type:Journal Article
- Keywords:
QAMS;
Xiao'er Huatan Zhike granules;
relative correction factor;
content determination
- From:
Drug Standards of China
2025;26(4):399-403
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a quantitative analysis of multi-components by single marker(QAMS)method for determination of cephaeline,emetine,ipecoside in Xiao'er Huatan Zhike granules.Methods:The column was Agilent Eclipse C18(4.6 mm ×250 mm,5 μm).The mobile phase was acetonitrile-0.1％phosphoric acid solu-tion with gradient elution at the flowrate of 1.0 mL·min-1.The column temperature was 35 ℃,and the detection wavelength was 203 nm.Taking cephaeline as the internal reference,the relative correction factors of emetine and ipecoside were calculated,and then the content determination were carried out.Results:The three components showed good linear relationships within their own ranges(r＞0.999 0),and the average recoveries were 96.78％-105.01％with the RSDs of 2.20％-3.24％.There were no significant differences between the values obtained by QAMS and those obtained by an external standard method.Conclusion:The established QAMS method can be used for simultaneously determining the contents of 3 alkaloids in Xiao'er Huatan Zhike granules.
