Relationship between Expression of ATP5B mRNA,UBA52 mRNA in Peripheral Blood and Renal Injury and Disease Recurrence in Children with IgA Vasculitis
10.3969/j.issn.1671-7414.2025.01.019
- VernacularTitle:IgA血管炎儿童外周血ATP5B mRNA,UBA52 mRNA表达与肾损伤及疾病复发的关系研究
- Author:
Xiaotao PAN
1
;
Hui TANG
1
Author Information
1. 上海市奉贤区中心医院检验科,上海 201499
- Publication Type:Journal Article
- Keywords:
immunoglobulin A vasculitis;
kidney injury;
ATP5B mRNA;
UBA52 mRNA
- From:
Journal of Modern Laboratory Medicine
2025;40(1):99-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the expression of ATP5B mRNA and UBA52 mRNA in the peripheral blood of children with IgA vasculitis(IgA) and kidney injury and disease recurrence. Methods 143 children with IgAV admitted to Shanghai Fengxian District Central Hospital from January 2022 to October 2023 were selected as the study subjects,and were further divided into IgAV kidney injury group(n=82)and IgAV kidney non-injury group (n=61)according to whether they were combined with the involvement of the kidneys . According to the postoperative follow-up,the patients were divided into a relapse group (n=44)and a non-recurrence group (n=99). In addition,60 cases of healthy medical checkups were selected as the control group in the same period. Real-time fluorescence quantitative PCR was used to detect the expression levels of ATP5B mRNA and UBA52mRNA and compared the expression levels of the two in each group. Multivariate Logistic regression analysis was performed to analyze the influencing factors of kidney injury in IgAV children. The predictive value of ATP5BmRNA and UBA52mRNA expression on renal injury and disease recurrence in IgAV children was analyzed by receiver operator characteristic (ROC) curve. Results The levels of ATP5BmRNA(1.01±0.21,1.24±0.26,1.46±0.35),UBA52 mRNA (1.03±0.19,1.19±0.22,1.42±0.28),Scr(55.69±12.78μmol/L,62.85±13.92μmol/L,76.49±15.34μmol/L) and BUN(11.85±2.91mmol/L,13.46±2.78mmol/L,17.54±3.45mmol/L) in peripheral blood of the control group,IgAV renal injury group and IgAV renal injury group were increased successively,and the differences between groups were statistically significant(F=39.666～64.417,all P<0.05). Compared with the non-relapsed group,the expressions of ATP5B mRNA (1.52±0.34 vs 1.19±0.20)and UBA52 mRNA (1.49±0.31 vs 1.08±0.21) in peripheral blood of the relapsed group were increased,and the differences were statistically significant (t=7.253,9.241,all P<0.001). Logistic regression analysis showed that,Scr,BUN,ATP5B and UBA52 were independent risk factors for the development of renal injury in IgAV (all P<0.05). The AUC(95％CI) of ATP5BmRNA combined with UBA52mRNA in peripheral blood was 0.823(0.607～0.914),which was significantly higher than the single prediction of both(Z=1.952,2.021,all P<0.05). The AUC(95％CI) of ATP5BmRNA combined with UBA52mRNA in peripheral blood to predict IgAV disease recurrence was 0.851(0.617～0.958),which was also significantly better than the single prediction of both(Z=2.035,2.098,all P<0.05). Conclusion The expression levels of ATP5B mRNA and UBA52 mRNA in peripheral blood of children with IgAV are associated with renal injury and recurrence of IgAV,which can be used as effective biomarkers for disease diagnosis and prognosis.
