Complete genomic sequence analysis of the G6P1bovine rotavirus BLL strain
10.3969/j.issn.1002-2694.2025.00.004
- VernacularTitle:G6P[1]型牛轮状病毒BLL株全基因组遗传特征分析
- Author:
Jin-hua ZHANG
1
;
Xia-fei LIU
;
Jun-jie YU
;
Jia-xin FAN
;
Ming-yue WANG
;
Guang-ping XIONG
;
Yi-peng WANG
;
Dan-di LI
;
Xiao-man SUN
;
Li-li PANG
;
Zhao-jun DUAN
Author Information
1. 甘肃中医药大学公共卫生学院,兰州 730000;传染病溯源预警与智能决策全国重点实验室,国家卫生健康委员会医学病毒和病毒病重点实验室,中国疾病预防控制中心病毒病预防控制所,北京 102206
- Publication Type:Journal Article
- Keywords:
rotavirus;
G6P[1];
sequence analysis;
VP8*protein;
geneticevolution
- From:
Chinese Journal of Zoonoses
2025;41(1):8-14
- CountryChina
- Language:Chinese
-
Abstract:
Bovine rotavirus(BRV)is an important pathogen causing diarrhea in calves.To understand the genomic charac-teristics and genetic variations in bovine rotavirus,and to further enrich data on the biological characteristics of rotavirus,we aimed to amplify 11 gene segments of the isolated and cultured G6P[1]bovine rotavirus BLL strain,perform whole genome se-quencing,and analyze the molecular characteristics.MEGA7.0 and DNAMAN software were used for homology and typing a-nalysis,and the whole genome phylogenetic tree was constructed to analyze genetic evolution relationships.The complete geno-type of the BLL strain was G6-P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3.Phylogenetic analysis of the VP7 and VP4 genes of the BLL strain showed that the VP7 gene had the highest homology with RVA/Cow-wt/HB01/China/2021,and the VP4 gene of the BLL strain was in the same branch as RVA/Human-tc/ISR/Ro8059/1995.From the sequence alignment of VP8*amino acids,the sialic acid domain of the BLL strain was found to be similar to that in other P[1]strains,but different from those in other types of strains,except for residue 189,which was the same as that in Ro8059 but different from that in other strains.The results suggested that the BLL strain might potentially infect humans.Therefore,continued monitoring and study of the biological characteristics of this strain are necessary to provide more information and evidence supporting further research on the cross-species transmission of group A rotavirus in China.
