Ring Finger 13 Alleviates Neuronal Ferroptosis in Cerebral Ischemia Reperfusion Injury by Stabilizing Ferritin Heavy Chain 1
10.3870/j.issn.1672-0741.25.03.006
- VernacularTitle:环指蛋白13通过稳定铁蛋白重链1减轻脑缺血再灌注损伤后神经元铁死亡
- Author:
Shuang LI
1
;
Qiangping WANG
;
Wenke ZHOU
Author Information
1. 华中科技大学同济医学院附属协和医院神经外科,武汉 430022
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion injury;
ferroptosis;
ring finger protein 13;
neuron;
ferritin
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(4):459-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the function of Ring finger protein 13(RNF13)in cerebral ischemia reperfusion injury(CIRI),and its mechanism.Methods The mouse middle cerebral artery embolization and primary neuron oxygen-glucose depri-vation and reoxygenation were used as disease models.The CRISPR/Cas9 gene knockout technique,immunohistochemical stai-ning,immunofluorescence staining,Western blot and lipid peroxidation detection were used to evaluate the regulatory effect and molecular mechanism of RNF13 on ferroptosis in CIRI.Student's t test was used for the comparison of two samples,and one-way analysis of variance was used for the comparison of multiple samples.Results The expression levels of RNF13 protein in mice and primary neurons were upregulated during CIRI.After knockout of RNF13,ferritin heavy chain 1(Fth1)and ferritin light chain(Ftl)were downregulated,and the content of free ferrous ions and the accumulation of lipid peroxides in mice brain tissues were promoted,leading to ferroptosis aggravation and neurological impairments.Overexpression of RNF13 protected a-gainst ferroptosis by reducing the production of free ferrous and lipid peroxides in neurons.Conclusion RNF13 alleviates fer-roptosis in neurons after CIRI,and the effect is induced by Fth1.
