In vivo effects of albendazole-glucan particles in the treatment of secondary Echinococcus metacestode infections in mice
10.3969/j.issn.1002-2694.2025.00.008
- VernacularTitle:阿苯达唑-葡聚糖纳米颗粒对小鼠继发性多房棘球蚴的疗效研究
- Author:
Hai-shan YANG
1
;
Sheng-xue LI
;
Hai-xia ZHANG
;
Hong-chang ZHAO
;
Tong-chuan XING
;
Xi-chen WU
;
Xiang ZHANG
;
Yan LIU
Author Information
1. 青海大学医学院,西宁 810016;青海大学附属医院,西宁 810001
- Publication Type:Journal Article
- Keywords:
Albendazole-glucan particles;
secondary alveolar coccosis;
targeting;
killing activity
- From:
Chinese Journal of Zoonoses
2025;41(1):15-22
- CountryChina
- Language:Chinese
-
Abstract:
Albendazole-glucan particles(ABZ-GPS)and abendazole(ABZ)were used to treat secondary alveolar echinococ-cosis in mice.The therapeutic effects of ABZ-GPS on alveolar echinococcosis in vivo were evaluated,and the feasibility of using glucan particles as anti-hydatid drug carriers was further verified.Mice with echinococcosis were randomly divided into an ABZ group,glucan nanoparticle(GP)group,ABZ-GPS group,and control group.After four courses of administration with a final concentration of 50 mg/mL,the therapeutic effects of ABZ-GPS were evaluated on the basis of imaging,histopathological changes,ultrastructure,and immunology.After ABZ-GPS and ABZ administration,clear liver lesion tissue necrosis and large numbers of infiltrating lymphocytes were observed.Significant differences in the average cyst wet weight(t=7.83,P＜0.05),were observed between the ABZ-GPS group and ABZ group.Imaging revealed that ABZ-GPs were targeted to liver tissue.Pa-thology and ultrastructure analyses demonstrated that the alveolar hydatid cells in the liver in the control group and GP group grew well,and the vesicles were large,filled with cystic fluid,and translucent or transparent;the cyst wall tension was high;no calcification was observed;the stratum corneum and germinal layer were clear;and more fertile capsules and different num-bers of protocephalospora were present in the liver.In the ABZ group,the capsular cavity collapsed,and showed partial necro-sis and lymphocyte infiltration.In the ABZ-GP group,the corneum and germinal layer of echinococcus vesicles were difficult to identify,and we observed bulbous necrosis,central calcification,fibrous tissue hyperplasia,inflammatory cell infiltration,coarser,shorter or absent microvilli of the germinal layer,nuclear shrinkage,dissolution or disappearance,clear expansion of cytoplasmic microtubules,and myelin-like or vacuole-like changes.Therefore,ABZ-GPs showed good targeting and killing ac-tivity in vivo in mice with secondary alveolar coccosis.
