Simultaneous determination of three components in compound glycyrrhizin tablets by ion-pair chromatographic method
10.19778/j.chp.2025.04.015
- VernacularTitle:离子对色谱法测定复方甘草酸苷片中3种组分的含量
- Author:
Guojun KUANG
1
;
Yijuan ZHU
1
;
Yanjun LONG
1
;
Haishun FANG
1
Author Information
1. 广州市药品检验所,广州 510160
- Publication Type:Journal Article
- Keywords:
compound glycyrrhizin tablets;
glycyrrhizin;
D,L-methionine;
glycine;
content determination;
CAD detector
- From:
Drug Standards of China
2025;26(4):430-434
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an ion-pair chromatographic method combined with an electrospray detector(CAD)for the simultaneous determination of glycyrrhizin,glycine and D,L-methionine in compound glycyrrhizin tablets.Methods:An Agilent Poroshell 120 SB-C18 column(2.1 mm ×250 mm,4 μm)was adopted.0.3％non-afluorovaleric acid(A)-acetonitrile(B)was used as the mobile phase with a gradient elution(0-8 min,100％A,8-15 min,82％A,and 15-30 min,40％A).The flow rate was 0.2 mL·min-1.The column temperature was set at 30 ℃.The detector of CAD was applied with collection frequency of 10 Hz,nebulization temperature of 35 ℃,filter:5.Results:The peaks of glycyrrhizin,glycine,and D,L-methionine were well separated by this method,and the recoveries of spiked samples were 100.4％,100.4％,and 100.1％.The results of the three bat-ches of samples were in the range of 95.0％-105.0％.Conclusion:The method is show well convenience,sensi-tivity and accurate for the quality control of compound glycyrrhizin tablets.
