Effect and Mechanism of the Baihuang Ganning Mixture Alleviating Cholestatic Hepatitis in the Mice Model
10.3870/j.issn.1004-0781.2025.09.002
- VernacularTitle:败黄肝宁合剂对淤胆型肝炎模型小鼠的治疗作用及其机制
- Author:
Quanwei YANG
1
;
Lei HUANG
1
;
Yu TIAN
1
;
Song HU
1
;
Hongfeng XU
1
Author Information
1. 武汉市第一医院药学部,武汉 430022
- Publication Type:Journal Article
- Keywords:
Baihuang Ganning mixture;
Ursodeoxycholic acid;
Hepatitis,cholestatic
- From:
Herald of Medicine
2025;44(9):1379-1384
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the therapeutic effect of Baihuang Ganning mixture on mice with cholestatic hepatitis model induced by α-naphthalene isothiocyanate(ANIT),and to explore the mechanism by which Baihuang Ganning mixture improves cholestatic hepatitis.Methods A cholestatic hepatitis model was established in mice by intragastric administration of ANIT(60 mg·kg-1).Sixty mice were randomly divided into six groups(n=10):normal control group,model control group,the ursodeoxycholic acid group,and the high,medium,and low-dose of Baihuang Ganning mixture groups.Parameters including bile flow rate,serum biochemical indices(ALT,AST,T-BiL,D-BiL,ALP and TBA),histopathological changes in liver tissue,hepatic biochemical markers(MDA,NO,Na+-K+-ATPase and SOD),and expression levels of TGF-β1,P-Smad2,and P-Smad3 were evaluated.Results Serum biochemical analysis revealed that,compared to the model control group,the high and medium-dose Baihuang Ganning mixture groups exhibited significant reductions in ALT,AST,T-BiL,D-BiL,ALP,and TBA levels(P<0.01),along with increased bile flow rate(P<0.01).No significant changes were observed in the low-dose group.Histopathological examination demonstrated that high and medium-dose Baihuang Ganning mixture markedly alleviated ANIT-induced bile duct injury,cholestasis,and hepatocyte injury.Compared to the ursodeoxycholic acid group,high and medium-dose Baihuang Ganning mixture significantly reduced hepatic MDA and NO levels,enhanced Na+-K+-ATPase and SOD activities(P<0.01),and downregulated the expression of TGF-β1,P-Smad2,and P-Smad3.Conclusions Baihuang Ganning mixture significantly improved ANIT-induced cholestatic hepatitis in mice.Baihuang Ganning mixture can regulate the metabolism of bile acids,inhibit the proliferation and activation of hepatic stellate cells(HSC)and the synthesis of collagen,and delay the occurrence of cholestatic hepatitis by inhibiting the expression levels of TGF-β1,P-Smad2 and P-Smad3 proteins.
