Scutellarin regulates cAMP/PKA/CREB signaling pathway to improve cognitive dysfunction in rats with post-traumatic stress disorder model
10.3969/j.issn.1000-484X.2025.09.028
- VernacularTitle:灯盏花乙素调节cAMP/PKA/CREB信号通路改善创伤后应激障碍模型大鼠认知功能障碍
- Author:
Qiaoyu HE
1
;
Yang YANG
1
Author Information
1. 河南护理职业学院基础医学部,安阳 455000
- Publication Type:Journal Article
- Keywords:
Scutellarin;
Post-traumatic stress disorder;
Cognitive dysfunction;
Cyclic adenosine monophosphate/protein ki-nase A/cAMP response element binding protein signaling pathway
- From:
Chinese Journal of Immunology
2025;41(9):2226-2231
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the influence of scutellarin(Scu)on cognitive dysfunction in rats with post-traumatic stress disorder(PTSD)by regulating the expression of cAMP/PKA/cAMP response element binding protein(CREB)signaling path-way.Methods:The rat model of PTSD was established by compound stress.The rats were grouped into model group(PTSD),low,medium and high doses scutellarin groups(Scu-L,M,H,5,25,50 mg/kg)and positive control group(fluoxetine,10 mg/kg),in ad-dition,rats that were normally cultured(without compound stress)were set as the blank control group(NC).Open field experiment and Morris water maze experiment were applied to test the rats'autonomous activity ability and learning and memory ability;the histo-pathological changes in the hippocampus of rats was observed by HE staining;TUNEL staining was applied to observe the apoptosis of neurons in rat hippocampus;immunohistochemistry was used to detect the expression of apoptosis-related protein Caspase-3;the con-tents of IL-1β,IL-6 and TNF-α in hippocampus were measured by ELISA;the levels of SOD,cAMP and MDA in the hippocampus were detected by the corresponding kit;the expressions of p-PKA,PKA,p-CREB and CREB proteins were detected by Western blot.Results:Compared with NC group,rats in PTSD group showed pathological changes such as neuron apoptosis and inflammatory cell infiltration,the autonomic activity,learning and memory,SOD activity,the level of cAMP,the protein expression of p-PKA/PKA,p-CREB/CREB decreased obviously,the apoptosis rate of neurons,the expression of Caspase-3 protein,the contents of IL-1β,IL-6,TNF-α and MDA increased obviously(P<0.05);compared with PTSD group,the pathological changes such as neuron apoptosis and inflammatory cell infiltration of rats in Scu-M,H groups and fluoxetine group were obviously improved,the autonomic activity,learn-ing and memory,SOD activity,the level of cAMP,the protein expressions of p-PKA/PKA,p-CREB/CREB increased obviously,the apoptosis rate of neurons,the expression of Caspase-3 protein,the contents of IL-1β,IL-6,TNF-α and MDA decreased obviously(P<0.05).Conclusion:Scu can inhibit inflammation,oxidative stress and neuronal apoptosis,and improve cognitive dysfunction in PTSD rats by activating the expression of cAMP/PKA/CREB signaling pathway.
