Exploring effect of Bufei Yiyang Huatan Formula on cigarette smoke induced oxidative stress damage of human bronchial epithelial cells based on Keap1-Nrf2-ARE pathway
10.3969/j.issn.1000-484X.2025.09.026
- VernacularTitle:基于Keap1-Nrf2-ARE通路探讨补肺益阳化痰方对烟草烟雾诱导的人支气管上皮细胞氧化应激损伤的影响
- Author:
Lijun LU
1
;
Yuanyuan YU
1
;
Yang ZHENG
1
Author Information
1. 武汉市中医医院肺系病科,武汉 430014
- Publication Type:Journal Article
- Keywords:
Keap1-Nrf2-ARE pathway;
Bufei Yiyang Huatan Formula;
Cigarette smoke;
Human bronchial epithelial cells;
Oxidative stress damage
- From:
Chinese Journal of Immunology
2025;41(9):2214-2219,2225
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the effects of Bufei Yiyang Huatan Formula on oxidative stress damage of human bronchial epithelial cells induced by cigarette smoke(CS)and Keap1-Nrf2-ARE pathway.Methods:CS induced human bronchial epithelial cells and drug-containing serum of the Bufei Yiyang Huatan Formula were constructed,and cells were grouped into Control group,model group(CS group),CS+BFYHTF drug containing serum low,medium and high concentration groups(CS+BFYHTF-L,CS+BFYHTF-M,CS+BFYHTF-H groups),CS+BFYHTF high concentration serum+Nrf2 inhibitor group(CS+BFYHTF-H+ML385 group).CCK-8 was used to detect cell viability;DCFH-DA probe was used to detect content of ROS.ELISA was performed to detect inflammatory factors and oxidative stress in supernatant.Flow cytometry was performed to measure cell apoptosis;Western blot was used to measure cell apoptosis and expressions of Keap1-Nrf2-ARE pathway related proteins.Results:Compared with Control group,CS group showed higher ROS,MDA,TNF-α,IL-6,IL-1β,apoptosis rate,and C-caspase3,C-caspase9 and Keap1 proteins,lower SOD,GSH-Px activities,and Nrf2 and HO-1 expressions(P<0.05).CS+BFYHTF-L,CS+BFYHTF-M and CS+BFYHTF-H groups showed lower ROS,MDA,TNF-α,IL-6,IL-1β,apoptosis rate,and C-caspase3,C-caspase9 and Keap1 proteins,higher SOD,GSH-Px activi-ties,and Nrf2 and HO-1 expressions than CS group(P<0.05).Compared with CS+BFYHTF-H group,CS+BFYHTF-H+ML385 group showed higher ROS,MDA,TNF-α,IL-6,IL-1β,apoptosis rate,and C-caspase3,C-caspase9 and Keap1 proteins,lower SOD,GSH-Px activities,and Nrf2 and HO-1 expressions(P<0.05).Conclusion:Bufei Yiyang Huatan Formula can improve oxidative stress damage of human bronchial epithelial cells induced by CS,which is related to the inhibition of Keap1-Nrf2-ARE pathway.
