Expression levels and clinical value of METTL3,METTL14,WTAP,ALKBH5,FTO and YTHDF2 in PBMC from rheumatoid arthritis patients
10.3969/j.issn.1000-484X.2025.09.005
- VernacularTitle:类风湿关节炎患者PBMC中METTL3、METTL14、WTAP、ALKBH5、FTO、YTHDF2表达水平及临床价值研究
- Author:
Qiuyun XIAO
1
;
Yujie GAO
1
;
Qing LUO
1
Author Information
1. 南昌大学第一附属医院,南昌 330006
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis;
m6A;
Methylation modification;
PBMC
- From:
Chinese Journal of Immunology
2025;41(9):2074-2080
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the level of N6-methyladenosine(m6A)methylation modifier regulator in peripheral blood mononuclear cell(PBMC)of patients with rheumatoid arthritis(RA),and to investigate its expression level and clinical significance in RA.Methods:PBMC from 50 RA patients and 36 healthy controls(HC)were examined by RT-qPCR to compare the mRNA expres-sion levels of m6A methylation modifier regulators(METTL3,METTL14,WTAP,ALKBH5,FTO,YTHDF2)on PBMC from RA and HC groups and their correlation with laboratory test data was analyzed.Results:①The expression levels of m6A methylation modifica-tion regulators on PBMC in peripheral blood were significantly lower in the RA group than in the HC group,and the differences were statistically significant;②The expression levels of METTL3,METTL14,WTAP and YTHDF2 on PBMC of RA patients were negatively correlated with rheumatoid factor;③The expression levels of YTDHF2 on PBMC of RA patients were negatively correlated with inflam-matory indexes CRP,ESR,N,N%,NLR and dNLR,and positively correlated with L%and LMR;the expression levels of WTAP on PBMC of RA patients were negatively correlated with N,N%,NLR and dNLR,and positively correlated with L%and LMR;④The expression level of YTDHF2 on PBMC of RA patients increased significantly after treatment,and the difference was statistically signifi-cant;⑤The expression levels of m6A methylation modifier regulators on PBMC of RA patients were positively correlated with each other;⑥Logistic regression analysis showed that the decreased expression levels of FTO and YTHDF2 in PBMC of RA patients were risk factors for the development of RA.Conclusion:The expression levels of m6A methylation-modified regulators on PBMC are gener-ally reduced in the RA group.m6A methylation-modified regulators are clearly correlated with inflammation levels,autoantibody pro-duction and disease severity,and can be used as an indicator to assess the severity and activity of the disease.
