Effect of tolerogenic dendritic cells on autophagy of synovial cells in collagen-induced arthritis rats
10.3969/j.issn.1000-484X.2025.09.004
- VernacularTitle:耐受性树突状细胞对胶原诱导性关节炎大鼠滑膜细胞自噬的影响
- Author:
Honghong LI
1
;
Xiufang WAN
1
;
Rui YUAN
1
;
Tiaoyu LONG
1
;
Yan ZHOU
1
;
Hongmei JIANG
1
Author Information
1. 贵州医科大学医学检验学院临床微生物学及免疫学教研室,贵阳 550004
- Publication Type:Journal Article
- Keywords:
Tolerogenic dendritic cells;
Collagen-induced arthritis;
Autophagy;
Rheumatoid arthritis
- From:
Chinese Journal of Immunology
2025;41(9):2067-2073,2080
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of tolerogenic dendritic cells(tolDC)on autophagy of synovial cells in collagen-induced arthritis(CIA)rats.Methods:Bone marrow mononuclear cells of rats were extracted and induced into tolDC using IL-4,GM-CSF and NF-κB oligonucleotide decoy,and loaded with BⅡC to become BⅡC-tolDC.SD rats were randomly divided into normal control group,CIA model group and BⅡC-tolDC intervention group,with 3 rats in each group.Normal female SD rats were immunized with bovine type Ⅱ collagen solution to construct CIA model.Rats in BⅡC-tolDC intervention group were infused with BⅡC-tolDC via tail vein on the 21st day after initial immunization for two weeks,arthritis indexes were recorded weekly.On the 35th day,the rats were sacrificed,and synovial histopathology of ankle joint of rats in each group were observed by HE staining;the number of osteo-clasts in cartilage of rats in each group were observed by TRAP staining.The number of autophagic of ankle synovial cells of rats in each group were observed by transmission electron microscopy.Levels of serum TNF-α and IL-1β of rats in each group were detected by ELISA.LC3,Beclin-1 and ATG5 proteins of synovial cells of ankle joints of rats in each group were detected by Immunohistochemical staining.Results:CIA rats were constructed successfully by immunization with bovine type Ⅱ collagen.BⅡC-tolDC intervention re-duced the arthritis index of CIA rats,inhibited synovial inflammation and abnormal proliferation of synovial tissue,improved joint bone and cartilage injury,and reduced the number of osteoclasts in cartilage tissue and the number of autophagosomes in synovial cells.At the same time,reduced levels of serum TNF-α,IL-1β,and protein expressions of LC3,Beclin-1 and ATG5 of synovial cell of CIA rats.Conclusion:BⅡC-tolDC may alleviate arthritis lesions of CIA rats by inhibiting synovial cell autophagy of CIA rats.
