Zotepine Improves Intestinal Injury in Rats with Severe Acute Pancreatitis Through NF-κB Pathway
10.11969/j.issn.1673-548X.2025.07.012
- VernacularTitle:佐替平通过NF-κB通路改善重症急性胰腺炎大鼠肠损伤的机制研究
- Author:
Yijing LIU
1
;
Yujie LIN
1
;
Ling ZHANG
1
Author Information
1. 730000 兰州大学第二医院(第二临床医学院)急救中心
- Publication Type:Journal Article
- Keywords:
Zotepine;
Acute pancreatitis;
Acute pancreatitis associated intestinal injury;
p-p65;
p65
- From:
Journal of Medical Research
2025;54(7):60-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Zotepine on intestinal damage associated with severe acute pancretitis(SAP)in rats treated with p65/p-p65-mediated NF-κB signaling pathway.Methods Eighteen SD male rats were randomly divided into Sham group,SAP group and Zotepine group.After intraperitoneal injection of 3％pentobarbital sodium to induce anesthesia,the rats in Sham group were given retrograde injection of 1ml/kg normal saline into the pancreatic bile duct after laparotomy.SAP group and Zotepine group were molded by retrograde injection of 3.5％sodium taurine cholate(1ml/kg)into the bile duct of the pancreas,and zotepine group was injected intraperitoneally with zotepine solution(3mg/kg)two hours before and after modeling,and serum and related tissue samples were collected 24hours after surgery.Biochemical kit and enzyme-linked immunosorbent assay(ELISA)were used to detect serum amylase,related inflammatory factors and endotoxin content.The levels of related proteins were detected by Western blot.The pathological changes of intestinal tissue were observed by optical microscope,and the distribution of p65 and p-p65 in intestinal tissue was detected by immu-nofluorescence.Results The pathological damage degree of intestinal tissue showed a decreasing trend under the intervention of zotepine.The levels of amylase,endotoxin and related inflammatory factors in the Zotepine group were significantly reduced compared with those in the SAP group(P＜0.05).Meanwhile,Western blot results indicated that the conversion of p65 to p-p65 was also inhibited.Conclu-sion Zotepine can improve intestinal injury induced by severe acute pancreatitis in rats by inhibiting NF-κB pathway.
