Network pharmacology and molecular docking analysis and animal experimental study of ligustilide regulating H-type blood vessels in prevention and treatment of osteoporosis
10.12360/CPB202407017
- VernacularTitle:藁本内酯调控H型血管防治骨质疏松症的网络药理学与分子对接分析及动物实验研究
- Author:
Kai WANG
1
;
Hao-nan WEN
;
Zhi-jing SONG
;
Yong-jia SONG
;
Min SONG
Author Information
1. 甘肃省人民医院骨科
- Publication Type:Journal Article
- Keywords:
osteoporosis;
ligustilide;
H-type blood vessels;
EGFR;
PI3K/Akt signaling pathway;
molecu-lar mechanism
- From:
Chinese Pharmacological Bulletin
2025;41(3):583-591
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the biological mechanism of ligustilide in the prevention and treatment of osteo-porosis by regulating H-type blood vessels,combined with animal experiments for verification,based on net-work pharmacology and molecular docking technology Methods The possible mechanism of ligustilide regu-lating H-type blood vessels to prevent osteoporosis was predicted by network pharmacology.Molecular docking technology was used to verify the binding ability of the core target EGFR to ligustilide.The rat model of osteo-porosis was established and divided into the sham group,model group,ligustilide high,medium and low dose(80,40,20 mg·kg-1)groups.The pathological changes of femur were observed by HE staining.The expressions of CD31,EMCN,OSX+and RUNX2+pro-tein in tibial metaphysis were detected by immunofluo-rescence.The expression of p-EGFR,p-PI3K and p-Akt protein was detected by Western blot.Results The results of network pharmacology showed that a total of 20 intersection targets were obtained.EGFR,PTGS2,ESR1 and ICAM1 were core targets,and mo-lecular docking showed that EGFR had a strong bind-ing ability with ligustilide.The signaling pathways of ligustilide in the prevention and treatment of osteoporo-sis by regulating the expression of H-type blood vessels were mainly enriched in PI3K-Akt,TNF,etc.Com-pared with the model group,ligustilide could signifi-cantly increase the number of trabecular bone and im-prove the destruction of bone microstructure.The ex-pression of CD31,EMCN,OSX+and RUNX2+signifi-cantly increased(P＜0.01,P＜0.05),the formation of H-type blood vessels were promoted,and the expres-sion of p-EGFR,p-PI3K and p-Akt significantly in-creased(P＜0.01,P＜0.05).Conclusions Ligusti-lide can increase the expression of H-type blood vessels in bone tissue of osteoporosis model rats,reduce the damage of bone trabecula and improve bone micro-structure effectively.EGFR-mediated PI3K/Akt signa-ling pathway may be the key way to exert its biological effects.
