AMPK/PGC-1α signal pathway coordinates HBO-induced oxidative stress response
10.3760/cma.j.issn.1009-6906.2019.02.006
- VernacularTitle:AMPK/PGC-1α信号通路对高压氧诱导的氧化应激反应的调节作用
- Author:
Yanpeng WEI
1
;
Bin PENG
1
;
Yuan YUAN
1
;
Guohua WANG
1
;
Zhenglin JIANG
1
Author Information
1. 江苏省南通,南通大学航海医学研究所
- Publication Type:Journal Article
- Keywords:
AMPK/PGC-1α signaling pathway;
Hyperbaric oxygen;
Oxidative stress;
Mouse;
Hepatocyte;
Mitochondria
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(2):100-103,115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the adaptive response of the organism to oxidative stress induced by HBO,through observation of the effects of HBO on mitochondrial DNA (Mt-DNA) copy number and the expression of anti-oxidative protein and possible mechanism involved.Methods Twenty-eight C57BL/6 mice were randomly divided into 5 groups:the control group (with atmospheric pressure air exposure),the HBO group (with 0.2 MPa exposure for 1,5,14 and 28 days) (or the 1-d HBO group,the 5-d HBO group,the 14-d HBO group and the 28-d HBO group.Following exposure,the animals were immediately executed and hepatic tissues were collected.When total protein and DNA in the liver tissue were harvested,Mt-DNA copy number was determined by RT-PCR.The expression levels of superoxide dismutase 2 (SOD2),mitochondrial transcription factor A (TFAM),uncoupling protein 2 (UCP2) and AMPK/PGC-1 a signaling pathway proteins were detected by Western blotting.Results Compared with that of the control group (1.00 ±0.19),the Mt-DNA copy numbers in the 1-d HBO group(O.61 ± 0.18),the 5-d HBO group(0.58 ± 0.01),the 14-d HBO group (0.74 ± 0.07) and the 28-d HBO group (0.71 ± 0.07) were significantly reduced (P ＜ 0.05),while the expression levels of SOD2,TFAM,UCP2 and AMPK/PGC-1αt were all obviously increased.Statistical significance could be noted,when comparisons were made between the groups.(P ＜ 0.05 or P ＜ 0.01).Conclusion The coordination of HBO-induced oxidative stress response through access of the enhancement of anti-oxidative capacity and decrease of Mt-DNA copy number via activating AMPK/PGC-1α signaling pathway might be the mechanism involved in HBO-induced oxidative stress response in mice.
